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Positive signature-tagged mutagenesis in Pseudomonas aeruginosa: tracking patho-adaptive mutations promoting airways chronic infection.
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- Author(s): Bianconi I;Bianconi I; Milani A; Cigana C; Paroni M; Levesque RC; Bertoni G; Bragonzi A
- Source:
PLoS pathogens [PLoS Pathog] 2011 Feb 03; Vol. 7 (2), pp. e1001270. Date of Electronic Publication: 2011 Feb 03.- Publication Type:
Journal Article; Research Support, Non-U.S. Gov't- Language:
English - Source:
- Additional Information
- Source: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101238921 Publication Model: Electronic Cited Medium: Internet ISSN: 1553-7374 (Electronic) Linking ISSN: 15537366 NLM ISO Abbreviation: PLoS Pathog Subsets: MEDLINE
- Publication Information: Original Publication: San Francisco, CA : Public Library of Science, c2005-
- Subject Terms: Adaptation, Biological/*genetics ; Mutagenesis/*physiology ; Pseudomonas Infections/*microbiology ; Pseudomonas aeruginosa/*genetics ; Pseudomonas aeruginosa/*pathogenicity ; Respiratory Tract Infections/*microbiology; Algorithms ; Animals ; Cells, Cultured ; Chronic Disease ; Gene Expression Profiling ; Gene Expression Regulation, Bacterial ; Humans ; Male ; Mice ; Mice, Inbred C57BL ; Mutation/physiology ; Pseudomonas Infections/complications ; Pseudomonas Infections/genetics ; Pseudomonas Infections/pathology ; Pseudomonas aeruginosa/isolation & purification ; Respiratory Tract Infections/etiology ; Respiratory Tract Infections/pathology ; Virulence/genetics ; Virulence Factors/genetics
- Abstract: The opportunistic pathogen Pseudomonas aeruginosa can establish life-long chronic infections in the airways of cystic fibrosis (CF) patients. Persistent lifestyle is established with P. aeruginosa patho-adaptive variants, which are clonal with the initially-acquired strains. Several reports indicated that P. aeruginosa adapts by loss-of-function mutations which enhance fitness in CF airways and sustain its clonal expansion during chronic infection. To validate this model of P. aeruginosa adaptation to CF airways and to identify novel genes involved in this microevolution, we designed a novel approach of positive-selection screening by PCR-based signature-tagged mutagenesis (Pos-STM) in a murine model of chronic airways infection. A systematic positive-selection scheme using sequential rounds of in vivo screenings for bacterial maintenance, as opposed to elimination, generated a list of genes whose inactivation increased the colonization and persistence in chronic airways infection. The phenotypes associated to these Pos-STM mutations reflect alterations in diverse aspects of P. aeruginosa biology which include lack of swimming and twitching motility, lack of production of the virulence factors such as pyocyanin, biofilm formation, and metabolic functions. In addition, Pos-STM mutants showed altered invasion and stimulation of immune response when tested in human respiratory epithelial cells, indicating that P. aeruginosa is prone to revise the interaction with its host during persistent lifestyle. Finally, sequence analysis of Pos-STM genes in longitudinally P. aeruginosa isolates from CF patients identified signs of patho-adaptive mutations within the genome. This novel Pos-STM approach identified bacterial functions that can have important clinical implications for the persistent lifestyle and disease progression of the airway chronic infection.
- References: Science. 1996 Jan 5;271(5245):64-7. (PMID: 8539601)
Proc Natl Acad Sci U S A. 1998 Jul 21;95(15):8922-6. (PMID: 9671780)
Science. 2001 May 11;292(5519):1099-102. (PMID: 11352063)
Infect Immun. 1996 Jan;64(1):37-43. (PMID: 8557368)
Cell Microbiol. 2007 Jan;9(1):40-53. (PMID: 16869828)
J Clin Invest. 2002 Feb;109(3):317-25. (PMID: 11827991)
Science. 2000 May 19;288(5469):1251-4. (PMID: 10818002)
Microb Pathog. 2002 Nov;33(5):211-23. (PMID: 12473436)
Nat Protoc. 2009;4(7):1073-81. (PMID: 19561590)
J Infect Dis. 2005 Aug 1;192(3):410-9. (PMID: 15995954)
Mol Microbiol. 1998 Oct;30(2):295-304. (PMID: 9791175)
Cell Microbiol. 2002 May;4(5):285-96. (PMID: 12064285)
Proc Natl Acad Sci U S A. 2006 May 30;103(22):8487-92. (PMID: 16687478)
Mol Microbiol. 2007 Jul;65(1):153-65. (PMID: 17581126)
J Bacteriol. 1997 Dec;179(23):7280-90. (PMID: 9393691)
Microbiology (Reading). 2007 May;153(Pt 5):1445-1454. (PMID: 17464058)
Infect Immun. 1994 Feb;62(2):596-605. (PMID: 8300217)
Infect Immun. 1998 Jan;66(1):43-51. (PMID: 9423837)
Infect Immun. 2000 Jan;68(1):24-9. (PMID: 10603364)
Trends Biotechnol. 2010 Aug;28(8):398-406. (PMID: 20647113)
Environ Microbiol. 2008 Oct;10(10):2773-84. (PMID: 18643926)
Microbiology (Reading). 2006 Nov;152(Pt 11):3261-3269. (PMID: 17074897)
J Clin Microbiol. 2004 Nov;42(11):5229-37. (PMID: 15528719)
Proc Natl Acad Sci U S A. 2006 May 30;103(22):8305-6. (PMID: 16717189)
Environ Microbiol. 2003 Dec;5(12):1294-308. (PMID: 14641575)
Mol Microbiol. 1998 Oct;30(2):393-404. (PMID: 9791183)
Science. 1995 Jul 21;269(5222):400-3. (PMID: 7618105)
Microbiol Mol Biol Rev. 1997 Jun;61(2):136-69. (PMID: 9184008)
J Bacteriol. 2004 Jun;186(12):3855-61. (PMID: 15175299)
Mol Microbiol. 2007 Apr;64(2):512-33. (PMID: 17493132)
PLoS One. 2009 Dec 23;4(12):e8439. (PMID: 20037649)
Appl Microbiol Biotechnol. 2010 May;86(5):1323-36. (PMID: 20336292)
Int J Med Microbiol. 2010 Dec;300(8):584-93. (PMID: 20951086)
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1534-6. (PMID: 306628)
Annu Rev Immunol. 1993;11:129-63. (PMID: 8476559)
Mol Microbiol. 2009 Feb;71(3):730-47. (PMID: 19054330)
Microbiol Rev. 1979 Mar;43(1):73-102. (PMID: 111024)
Proc Natl Acad Sci U S A. 1997 Oct 28;94(22):12088-93. (PMID: 9342367)
Infect Immun. 1995 Jan;63(1):116-21. (PMID: 7806346)
Nat Rev Microbiol. 2004 Sep;2(9):747-65. (PMID: 15372085)
PLoS One. 2008 Feb 27;3(2):e1685. (PMID: 18301762)
J Cyst Fibros. 2009 Jan;8(1):66-70. (PMID: 18974024)
J Microbiol Methods. 2000 Aug;41(3):195-9. (PMID: 10958964)
Nat Immunol. 2002 Nov;3(11):1026-32. (PMID: 12407411)
Am J Respir Crit Care Med. 2009 Jul 15;180(2):138-45. (PMID: 19423715)
J Biol Chem. 1994 Aug 5;269(31):19787-95. (PMID: 8051059) - Grant Information: Canada Canadian Institutes of Health Research
- Accession Number: 0 (Virulence Factors)
- Publication Date: Date Created: 20110210 Date Completed: 20110610 Latest Revision: 20211020
- Publication Date: 20240628
- Accession Number: PMC3033382
- Accession Number: 10.1371/journal.ppat.1001270
- Accession Number: 21304889
- Source:
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