[The characteristics of phallotoxin-specific antibodies studied by immunization with a phallacidin-concanavalin A conjugate].

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Author(s): Khalacheva K; Zhelev Zh; Evrev T
  • Source:
    Eksperimentalna meditsina i morfologiia [Eksp Med Morfol] 1990; Vol. 29 (3), pp. 26-30.
  • Publication Type:
    English Abstract; Journal Article
  • Language:
    Bulgarian
  • Additional Information
    • Transliterated Title:
      Prouchvane i kharakteristika na falotoksinspetsifichni antitela posredstvom imunizatsiia s falatsidin-konkanavalin A koniugat.
    • Source:
      Publisher: Meditsina I Fizkultura Country of Publication: Bulgaria NLM ID: 0007506 Publication Model: Print Cited Medium: Print ISSN: 0367-0643 (Print) Linking ISSN: 03670643 NLM ISO Abbreviation: Eksp Med Morfol Subsets: MEDLINE
    • Publication Information:
      Publication: Sofia : Meditsina I Fizkultura
      Original Publication: Sofiia.
    • Subject Terms:
      Antibody Specificity*; Amanitins/*immunology ; Antibodies/*analysis ; Concanavalin A/*immunology ; Immunization/*methods ; Peptides, Cyclic/*immunology; Animals ; Chromatography, Affinity/methods ; Chromatography, Gel/methods ; Immunodiffusion/methods ; Male ; Rabbits
    • Abstract:
      High titre antiserum was obtained by immunization of rabbits with phalacidine-concanavaline A. Specific antiphalacidine antibodies were isolated from this serum on immobilized phalacidine. It was established by testing with antirabbit globulin sera that these antibodies were of IgG class only. IgG fraction was isolated from the immune serum by ion-exchange chromatography, but specific antibodies, which represented 8% of IgG of the serum, were isolated from it by affinity chromatography. The antibodies isolated by affinity, were tested against other phalatoxines and amotoxines. The results showed that they reacted with phalatoxines, but not with amatoxines and could be used in tests for quantitative determination of phalatoxines in mushroom extracts and body fluids.
    • Accession Number:
      0 (Amanitins)
      0 (Antibodies)
      0 (Peptides, Cyclic)
      11028-71-0 (Concanavalin A)
      26645-35-2 (phallacidin)
      54351-96-1 (phallotoxin)
    • Publication Date:
      Date Created: 19900101 Date Completed: 19910301 Latest Revision: 20091111
    • Publication Date:
      20221213
    • Accession Number:
      2125936