Is the intestine the sole source of heparin-stimulated plasma diamine oxidase? Acute effects of jejunectomy, ileectomy and total enterectomy.

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  • Additional Information
    • Source:
      Publisher: Karger Country of Publication: Switzerland NLM ID: 0150472 Publication Model: Print Cited Medium: Print ISSN: 0012-2823 (Print) Linking ISSN: 00122823 NLM ISO Abbreviation: Digestion Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel, Karger.
    • Subject Terms:
      Amine Oxidase (Copper-Containing)/*metabolism ; Heparin/*pharmacology ; Ileum/*surgery ; Intestinal Mucosa/*enzymology ; Jejunum/*surgery; Amine Oxidase (Copper-Containing)/blood ; Animals ; Ileum/enzymology ; Jejunum/enzymology ; Male ; Rats ; Rats, Inbred Strains ; Time Factors
    • Abstract:
      The assumption that the intestine is the sole/major source of post-heparin diamine oxidase (DAO) has been tested only once by Kobayashi et al. in 1969. In this study we tested whether the intestine is indeed the sole/major source of post-heparin plasma DAO and furthermore we studied the relative contribution of jejunum and ileum to post-heparin plasma DAO. We measured non-fasting plasma DAO before and every 15 min for 2 h after i.v. heparin (4,000 U/kg BW) and compared the resultant areas under the concentration-time curves (AUCs: mU/l X 2 h) in unoperated controls, jejunectomised, ileectomised and totally enterectomised (from gastro-oesophageal to colorectal junctions) male Wistar rats. Total enterectomy virtually abolished the post-heparin rise in plasma DAO, the mean AUC falling from 55.3 +/- (SEM) 10.2 in controls to 2.1 +/- 1.1 after enterectomy (p less than 0.001). The mean AUCs after jejunectomy and ileectomy were 25.6 +/- 5.5 and 10.75 +/- 2.42, respectively. Therefore in acute jejunal and ileal resection the AUCs were 2.1 and 5.4 times, respectively, lower than those seen in the controls (p less than 0.02 and p less than 0.001). The difference in mean AUC values between jejunectomy and ileectomy groups was also statistically significant (p less than 0.05). We conclude that total enterectomy abolishes the post-heparin rise in circulating DAO activity, confirming that the intestine is the major source of post-heparin plasma DAO. The ileum contributes more than the jejunum to this post-heparin rise in plasma enzyme activity.
    • Accession Number:
      9005-49-6 (Heparin)
      EC 1.4.3.21 (Amine Oxidase (Copper-Containing))
    • Publication Date:
      Date Created: 19900101 Date Completed: 19910207 Latest Revision: 20180216
    • Publication Date:
      20240829
    • Accession Number:
      10.1159/000200419
    • Accession Number:
      2124565