Single oral dose of micellar β-carotene containing phospholipids improves β-carotene metabolism and plasma lipids in vitamin A-deficient rats.

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  • Author(s): Marisiddaiah R;Marisiddaiah R; Rangaswamy L; Vallikannan B
  • Source:
    European journal of nutrition [Eur J Nutr] 2011 Oct; Vol. 50 (7), pp. 531-41. Date of Electronic Publication: 2010 Dec 25.
  • Publication Type:
    Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Steinkopff Country of Publication: Germany NLM ID: 100888704 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1436-6215 (Electronic) Linking ISSN: 14366207 NLM ISO Abbreviation: Eur J Nutr Subsets: MEDLINE
    • Publication Information:
      Original Publication: Darmstadt, Germany : Steinkopff, 1999-
    • Subject Terms:
      Micelles*; Lysophosphatidylcholines/*blood ; Phosphatidylcholines/*blood ; Vitamin A/*analogs & derivatives ; beta Carotene/*administration & dosage ; beta Carotene/*blood; Animals ; Diet ; Diterpenes ; Dose-Response Relationship, Drug ; Lipid Metabolism/drug effects ; Liver/chemistry ; Liver/drug effects ; Male ; Rats ; Rats, Wistar ; Retinyl Esters ; Vitamin A/blood ; Vitamin A Deficiency/drug therapy
    • Abstract:
      Background: Vitamin A (VA) deficiency is still a major health problem in the developing world. It affects various cellular functions and causes hypolipidemic effects in the body. β-Carotene (BC)-rich foods are promising sources of VA. Phospholipids are reported to improve BC bioefficacy in normal rats, but whether they show similar effects during VA deficiency is unknown.
      Aim: To compare the BC metabolism and plasma lipid responses in VA-sufficient (+VA) and VA-deficient (-VA) rats after a single oral dose of micellar BC containing phospholipids.
      Methods: Groups of rats were fed with a VA-free diet and when they attained the weight-plateau stage of deficiency, both +VA and -VA rats were divided into 2 groups (phosphatidylcholine, PC and lysophosphatidylcholine, LPC). Each group was further divided into 4 subgroups (1, 2, 3, and 6 h; n = 5 rats/time point) and determined the BC metabolism and plasma lipid responses to a post-dose of micellar BC with phospholipids.
      Results: Maximal plasma BC (pmol/mL) levels were observed at 2 h in PC (1330 ± 124) and at 1 h in LPC (1576 ± 144) groups of +VA rats, and at 3 h in the PC (1621 ± 158) and LPC (2248 ± 675) groups of -VA rats. Liver BC (pmol/g) was maximum at 1 h in the PC (218 ± 32) and LPC (249 ± 24) groups of +VA rats, and at 2 h in PC (228 ± 23) and at 3 h in LPC (277 ± 18) groups of -VA rats. Plasma and liver BC levels were significantly (P < 0.05) higher in -VA rats than +VA rats. Plasma retinyl palmitate (pmol/mL) was maximum at 3 h in PC (97 ± 18) and at 2 h in LPC (126 ± 14) groups of +VA rats, and at 2 h in the PC (92 ± 13) and LPC (134 ± 27) groups of -VA rats. The higher (P < 0.05) BC monoxygenase activity in -VA rats compared to +VA rats supports the BC bioefficacy. Plasma retinol level was improved in the PC and LPC groups, but the effect of LPC was higher (P < 0.05) than PC. Micellar phospholipids mitigate the VA deficiency-induced hypolipidemic effects.
      Conclusions: Micellar phospholipids improved BC metabolism and reinstated the hypolipidemic effects, perhaps by modifying the fat-metabolizing enzymes and repairing the altered intestinal membrane structure.
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    • Accession Number:
      0 (Diterpenes)
      0 (Lysophosphatidylcholines)
      0 (Micelles)
      0 (Phosphatidylcholines)
      0 (Retinyl Esters)
      01YAE03M7J (beta Carotene)
      11103-57-4 (Vitamin A)
      1D1K0N0VVC (retinol palmitate)
    • Publication Date:
      Date Created: 20101229 Date Completed: 20120130 Latest Revision: 20211020
    • Publication Date:
      20221213
    • Accession Number:
      10.1007/s00394-010-0160-5
    • Accession Number:
      21188595