mRNA for the EAAC1 subtype of glutamate transporter is present in neuronal dendrites in vitro and dramatically increases in vivo after a seizure.

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  • Author(s): Ross JR;Ross JR; Porter BE; Buckley PT; Eberwine JH; Robinson MB
  • Source:
    Neurochemistry international [Neurochem Int] 2011 Feb; Vol. 58 (3), pp. 366-75. Date of Electronic Publication: 2010 Dec 24.
  • Publication Type:
    Journal Article; Research Support, N.I.H., Extramural
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Pergamon Press Country of Publication: England NLM ID: 8006959 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-9754 (Electronic) Linking ISSN: 01970186 NLM ISO Abbreviation: Neurochem Int Subsets: MEDLINE
    • Publication Information:
      Original Publication: Oxford [Elmsford, N. Y.] Pergamon Press.
    • Subject Terms:
      Up-Regulation*/genetics; Dendrites/*metabolism ; Epilepsy/*metabolism ; Excitatory Amino Acid Transporter 3/*genetics ; Glutamic Acid/*genetics ; RNA, Messenger/*biosynthesis; Animals ; Cell Polarity/genetics ; Cells, Cultured ; Dendrites/pathology ; Disease Models, Animal ; Epilepsy/genetics ; Epilepsy/pathology ; Glutamic Acid/biosynthesis ; Rats ; Rats, Sprague-Dawley
    • Abstract:
      The neuronal Na(+)-dependent glutamate transporter, excitatory amino acid carrier 1 (EAAC1, also called EAAT3), has been implicated in the control of synaptic spillover of glutamate, synaptic plasticity, and the import of cysteine for neuronal synthesis of glutathione. EAAC1 protein is observed in both perisynaptic regions of the synapse and in neuronal cell bodies. Although amino acid residues in the carboxyl terminal tail have been implicated in the dendritic targeting of EAAC1 protein, it is not known if mRNA for EAAC1 may also be targeted to dendrites. Sorting of mRNA to specific cellular domains provides a mechanism by which signals can rapidly increase translation in a local environment; this form of regulated translation has been linked to diverse biological phenomena ranging from establishment of polarity during embryogenesis to synapse development and synaptic plasticity. In the present study, EAAC1 mRNA sequences were amplified from dendritic samples that were mechanically harvested from low-density hippocampal neuronal cultures. In parallel analyses, mRNA for histone deacetylase 2 (HDAC-2) and glial fibrillary acidic protein (GFAP) was not detected, suggesting that these samples are not contaminated with cell body or glial mRNAs. EAAC1 mRNA also co-localized with Map2a (a marker of dendrites) but not Tau1 (a marker of axons) in hippocampal neuronal cultures by in situ hybridization. In control rats, EAAC1 mRNA was observed in soma and proximal dendrites of hippocampal pyramidal neurons. Following pilocarpine- or kainate-induced seizures, EAAC1 mRNA was present in CA1 pyramidal cell dendrites up to 200μm from the soma. These studies provide the first evidence that EAAC1 mRNA localizes to dendrites and suggest that dendritic targeting of EAAC1 mRNA is increased by seizure activity and may be regulated by neuronal activity/depolarization.
      (Copyright © 2010 Elsevier Ltd. All rights reserved.)
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    • Grant Information:
      P30 HD026979 United States HD NICHD NIH HHS; R01 NS056222 United States NS NINDS NIH HHS; R37 AG009900 United States AG NIA NIH HHS; R01 NS056222-04 United States NS NINDS NIH HHS; R37 AG009900-20 United States AG NIA NIH HHS; R01 HD060132-17 United States HD NICHD NIH HHS; R01 HD060132 United States HD NICHD NIH HHS; R01 AG009900 United States AG NIA NIH HHS; F31 NS049690-05 United States NS NINDS NIH HHS; P30 HD26979 United States HD NICHD NIH HHS; F31 NS049690 United States NS NINDS NIH HHS; R01 AG9900 United States AG NIA NIH HHS; P30 HD026979-21 United States HD NICHD NIH HHS
    • Accession Number:
      0 (Excitatory Amino Acid Transporter 3)
      0 (RNA, Messenger)
      0 (Slc1a1 protein, rat)
      3KX376GY7L (Glutamic Acid)
    • Publication Date:
      Date Created: 20101228 Date Completed: 20111227 Latest Revision: 20211020
    • Publication Date:
      20240829
    • Accession Number:
      PMC3040252
    • Accession Number:
      10.1016/j.neuint.2010.12.012
    • Accession Number:
      21185901