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Comparison of recombinant protein expression in a baculovirus system in insect cells (Sf9) and silkworm.
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- Additional Information
- Source:
Publisher: Oxford University Press Country of Publication: England NLM ID: 0376600 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1756-2651 (Electronic) Linking ISSN: 0021924X NLM ISO Abbreviation: J Biochem Subsets: MEDLINE
- Publication Information:
Publication: : Abingdon, UK : Oxford University Press
Original Publication: Tokyo : Japanese Biochemical Society
- Subject Terms:
- Abstract:
Using a hybrid baculovirus system, we compared the expression of 45 recombinant proteins from six categories using two models: silkworm (larvae and pupae) and an Sf9 cell line. A total of 45 proteins were successfully expressed; preparation of hybrid baculovirus was unsuccessful for one protein, and two proteins were not expressed. A similar pattern of expression was seen in both silkworm and Sf9 cells, with double and multiple bands found in immunoblotting of the precipitate of both hosts. Degraded proteins were seen only in the silkworm system (particularly in the larvae). Production was more efficient in silkworms; a single silkworm produced about 70 times more protein than 10(6) Sf9 cells in 2 ml of culture medium.
- Accession Number:
0 (Peptide Fragments)
0 (Recombinant Proteins)
- Publication Date:
Date Created: 20101130 Date Completed: 20110629 Latest Revision: 20110121
- Publication Date:
20221213
- Accession Number:
10.1093/jb/mvq138
- Accession Number:
21113054
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