The effect of spider toxin PhTx3-4, ω-conotoxins MVIIA and MVIIC on glutamate uptake and on capsaicin-induced glutamate release and [Ca2+]i in spinal cord synaptosomes.

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  • Additional Information
    • Source:
      Publisher: Springer Country of Publication: Netherlands NLM ID: 8200709 Publication Model: Print Cited Medium: Internet ISSN: 1573-6830 (Electronic) Linking ISSN: 02724340 NLM ISO Abbreviation: Cell Mol Neurobiol Subsets: MEDLINE
    • Publication Information:
      Publication: 1981- : [Dordrecht, Netherlands] : Springer
      Original Publication: New York : Plenum Press, c1981-
    • Subject Terms:
      Calcium/*metabolism ; Capsaicin/*pharmacology ; Glutamic Acid/*metabolism ; Neuropeptides/*toxicity ; Spinal Cord/*metabolism ; Synaptosomes/*metabolism ; omega-Conotoxins/*toxicity; Animals ; Fluorescence ; Male ; Rats ; Rats, Wistar ; Spider Venoms/toxicity ; Spinal Cord/drug effects ; Synaptosomes/drug effects
    • Abstract:
      In spinal cord synaptosomes, the spider toxin PhTx3-4 inhibited capsaicin-stimulated release of glutamate in both calcium-dependent and -independent manners. In contrast, the conus toxins, ω-conotoxin MVIIA and xconotoxin MVIIC, only inhibited calcium-dependent glutamate release. PhTx3-4, but not ω-conotoxin MVIIA or xconotoxin MVIIC, is able to inhibit the uptake of glutamate by synaptosomes, and this inhibition in turn leads to a decrease in the Ca(2+)-independent release of glutamate. No other polypeptide toxin so far described has this effect. PhTx3-4 and ω-conotoxins MVIIC and MVIIA are blockers of voltage-dependent calcium channels, and they significantly inhibited the capsaicin-induced rise of intracellular calcium [Ca(2+)](i) in spinal cord synaptosomes, which likely reflects calcium entry through voltage-gated calcium channels. The inhibition of the calcium-independent glutamate release by PhTx3-4 suggests a potential use of the toxin to block abnormal glutamate release in pathological conditions such as pain.
    • Accession Number:
      0 (Neuropeptides)
      0 (Spider Venoms)
      0 (Tx3 neurotoxin)
      0 (omega-Conotoxins)
      147794-23-8 (omega-conotoxin-MVIIC)
      3KX376GY7L (Glutamic Acid)
      7I64C51O16 (ziconotide)
      S07O44R1ZM (Capsaicin)
      SY7Q814VUP (Calcium)
    • Publication Date:
      Date Created: 20101110 Date Completed: 20110718 Latest Revision: 20241024
    • Publication Date:
      20241024
    • Accession Number:
      10.1007/s10571-010-9618-5
    • Accession Number:
      21061150