Construction of an integrated genetic linkage map for the A genome of Brassica napus using SSR markers derived from sequenced BACs in B. rapa.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read Online Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2164 (Electronic) Linking ISSN: 14712164 NLM ISO Abbreviation: BMC Genomics Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : BioMed Central, [2000-
    • Subject Terms:
      Genetic Linkage*; Brassica napus/*genetics ; Brassica rapa/*genetics ; Chromosome Mapping/*methods ; Chromosomes, Artificial, Bacterial/*genetics ; Genome, Plant/*genetics ; Minisatellite Repeats/*genetics; Base Sequence ; Contig Mapping ; Genetic Markers ; Reproducibility of Results ; Seeds/genetics ; Sequence Analysis, DNA
    • Abstract:
      Background: The Multinational Brassica rapa Genome Sequencing Project (BrGSP) has developed valuable genomic resources, including BAC libraries, BAC-end sequences, genetic and physical maps, and seed BAC sequences for Brassica rapa. An integrated linkage map between the amphidiploid B. napus and diploid B. rapa will facilitate the rapid transfer of these valuable resources from B. rapa to B. napus (Oilseed rape, Canola).
      Results: In this study, we identified over 23,000 simple sequence repeats (SSRs) from 536 sequenced BACs. 890 SSR markers (designated as BrGMS) were developed and used for the construction of an integrated linkage map for the A genome in B. rapa and B. napus. Two hundred and nineteen BrGMS markers were integrated to an existing B. napus linkage map (BnaNZDH). Among these mapped BrGMS markers, 168 were only distributed on the A genome linkage groups (LGs), 18 distrubuted both on the A and C genome LGs, and 33 only distributed on the C genome LGs. Most of the A genome LGs in B. napus were collinear with the homoeologous LGs in B. rapa, although minor inversions or rearrangements occurred on A2 and A9. The mapping of these BAC-specific SSR markers enabled assignment of 161 sequenced B. rapa BACs, as well as the associated BAC contigs to the A genome LGs of B. napus.
      Conclusion: The genetic mapping of SSR markers derived from sequenced BACs in B. rapa enabled direct links to be established between the B. napus linkage map and a B. rapa physical map, and thus the assignment of B. rapa BACs and the associated BAC contigs to the B. napus linkage map. This integrated genetic linkage map will facilitate exploitation of the B. rapa annotated genomic resources for gene tagging and map-based cloning in B. napus, and for comparative analysis of the A genome within Brassica species.
    • References:
      Nat Genet. 2002 Feb;30(2):194-200. (PMID: 11799393)
      Plant Cell. 2006 Jun;18(6):1339-47. (PMID: 16632644)
      BMC Plant Biol. 2010 Mar 29;10:54. (PMID: 20350303)
      Genome. 1995 Dec;38(6):1122-31. (PMID: 18470236)
      Trends Biotechnol. 2005 Jan;23(1):48-55. (PMID: 15629858)
      Theor Appl Genet. 2009 Dec;120(1):31-43. (PMID: 19784615)
      Genetics. 2006 Sep;174(1):29-39. (PMID: 16988107)
      Genome Res. 2000 Jun;10(6):776-88. (PMID: 10854410)
      BMC Genomics. 2008 Jun 12;9:280. (PMID: 18549474)
      Genome. 2007 Jul;50(7):611-8. (PMID: 17893738)
      Genetics. 2006 May;173(1):309-19. (PMID: 16723420)
      Methods Mol Biol. 2000;132:365-86. (PMID: 10547847)
      Mol Cells. 2007 Jun 30;23(3):349-56. (PMID: 17646709)
      Theor Appl Genet. 2005 Nov;111(8):1514-23. (PMID: 16187118)
      Theor Appl Genet. 1996 Sep;93(4):534-8. (PMID: 24162344)
      Genome Res. 2001 Aug;11(8):1441-52. (PMID: 11483586)
      Ann Bot. 2005 Jan;95(1):229-35. (PMID: 15596470)
      Genome Res. 2005 Apr;15(4):516-25. (PMID: 15781573)
      Theor Appl Genet. 2008 Oct;117(6):977-85. (PMID: 18651126)
      Genome Biol. 2001;2(3):REVIEWS1011. (PMID: 11276431)
      Theor Appl Genet. 2002 May;104(6-7):1092-1098. (PMID: 12582617)
      Genome Res. 2005 Apr;15(4):487-95. (PMID: 15805490)
      New Phytol. 2010 Apr;186(1):102-12. (PMID: 20149113)
      Genome. 2008 Mar;51(3):169-76. (PMID: 18356952)
      Genome. 2010 Jan;53(1):55-67. (PMID: 20130749)
      Mol Biol Evol. 2001 Jul;18(7):1161-7. (PMID: 11420357)
      BMC Genomics. 2009 Sep 15;10:432. (PMID: 19751531)
      Plant Cell. 2009 Jul;21(7):1912-28. (PMID: 19602626)
      Theor Appl Genet. 2007 Oct;115(6):777-92. (PMID: 17646962)
      Genetics. 2005 Oct;171(2):765-81. (PMID: 16020789)
      BMC Genomics. 2008 Mar 03;9:113. (PMID: 18315867)
      Theor Appl Genet. 2004 Apr;108(6):1103-12. (PMID: 15067397)
      Theor Appl Genet. 2009 Apr;118(6):1121-31. (PMID: 19190889)
      Comp Funct Genomics. 2005;6(3):138-46. (PMID: 18629219)
      Int J Plant Genomics. 2008;2008:582837. (PMID: 18288250)
    • Accession Number:
      0 (Genetic Markers)
    • Publication Date:
      Date Created: 20101026 Date Completed: 20101227 Latest Revision: 20220310
    • Publication Date:
      20231215
    • Accession Number:
      PMC3091739
    • Accession Number:
      10.1186/1471-2164-11-594
    • Accession Number:
      20969760