[Effect of dimethyladipimidate and dimethylsuberimidate on cell-cell adhesion in rat fibroblasts].

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  • Author(s): Pippia P;Pippia P; Cogoli A; Sciola L; Tilloca G; Meloni MA; Barni S
  • Source:
    Bollettino della Societa italiana di biologia sperimentale [Boll Soc Ital Biol Sper] 1990 Oct; Vol. 66 (10), pp. 945-52.
  • Publication Type:
    Comparative Study; English Abstract; Journal Article
  • Language:
    Italian
  • Additional Information
    • Transliterated Title:
      Effetto del dimetiladipimidato e del dimetilsuberimidato sull'adesione cellula-cellula di fibroblasti di ratto.
    • Source:
      Publisher: PAGEPress Country of Publication: Italy NLM ID: 7506962 Publication Model: Print Cited Medium: Print ISSN: 0037-8771 (Print) Linking ISSN: 00378771 NLM ISO Abbreviation: Boll Soc Ital Biol Sper Subsets: MEDLINE
    • Publication Information:
      Publication: 2014- : Pavia, Italy : PAGEPress
      Original Publication: Napoli, Società italiana di biologia sperimentale.
    • Subject Terms:
      Cell Adhesion/*drug effects ; Cross-Linking Reagents/*pharmacology ; Dimethyl Adipimidate/*pharmacology ; Dimethyl Suberimidate/*pharmacology ; Fibroblasts/*drug effects; Animals ; Cells, Cultured ; Fibroblasts/cytology ; Rats
    • Abstract:
      In a study performed to identify the molecular mechanisms which regulate cell to cell adhesion and contact inhibition in neoplastic and syngeneic normal cells of the rat we have observed that the adhesive capacity depends on the reagents used, either EDTA or trypsin, to release the cells from monolayer. Taking profit of this last property and of the possibility of blocking free -NH2 groups on membrane proteins with specific cross-linking reagents "in vitro", we have studied in this work the behaviour of the proteins of the cell coat involved in cell to cell adhesion of rat fibroblasts FG/2. The cross-linking reagents used were dimethyladipimidate (DMA) and dimethylsuberimidate (DMS). The cells were exposed to the reagents at 0 degrees C for 30'. Cell to cell adhesion was measured by determining the percentage of single cells labeled with 3H-leucine, adhering to a confluent monolayer at different incubation times. The inhibitory effect on cell to cell adhesion brought about by cross-linking reagents indicates that a) EDTA-released cells are more sensitive to both imides than those released with trypsin, b) DMA is more effective on trypsin-released cells and c) DMS is more effective on EDTA-released cells. Therefore, we conclude that the inhibition of adhesion by reaction with the two cross-linking reagents is more likely due to a stiffening of the molecules of the cell coat involved in the adhesion, rather than to the modification of -NH2 residues which should specifically participate to adhesive process.
    • Contributed Indexing:
      Indexing Agency: NASA Local ID #: 91265041.
    • Accession Number:
      0 (Cross-Linking Reagents)
      13139-70-3 (Dimethyl Adipimidate)
      29878-26-0 (Dimethyl Suberimidate)
    • Publication Date:
      Date Created: 19901001 Date Completed: 19910724 Latest Revision: 20061115
    • Publication Date:
      20240829
    • Accession Number:
      2096879