Genome nucleotide composition shapes variation in simple sequence repeats.

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  • Author(s): Tian X;Tian X; Strassmann JE; Queller DC
  • Source:
    Molecular biology and evolution [Mol Biol Evol] 2011 Feb; Vol. 28 (2), pp. 899-909. Date of Electronic Publication: 2010 Oct 13.
  • Publication Type:
    Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Oxford University Press Country of Publication: United States NLM ID: 8501455 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1537-1719 (Electronic) Linking ISSN: 07374038 NLM ISO Abbreviation: Mol Biol Evol Subsets: MEDLINE
    • Publication Information:
      Publication: 2003- : New York, NY : Oxford University Press
      Original Publication: [Chicago, Ill.] : University of Chicago Press, [c1983-
    • Subject Terms:
      Genome, Protozoan* ; Microsatellite Repeats*; DNA, Protozoan/*chemistry ; Eukaryota/*genetics; Base Composition
    • Abstract:
      Simple sequence repeats (SSRs) or microsatellites are a common component of genomes but vary greatly across species in their abundance. We tested the hypothesis that this variation is due in part to AT/GC content of genomes, with genomes biased toward either high AT or high CG generating more short random repeats that are long enough to enhance expansion through slippage during replication. To test this hypothesis, we identified repeats with perfect tandem iterations of 1-6 bp from 25 protists with complete or near-complete genome sequences. As expected, the density and the frequency are highly related to genome AT content, with excellent fits to quadratic regressions with minima near a 50% AT content and rising toward both extremes. Within species, the same trends hold, except the limited variation in AT content within each species places each mainly on the descending (GC rich), middle, or ascending (AT rich) part of the curve. The base usages of repeat motifs are also significantly correlated with genome nucleotide compositions: Percentages of AT-rich motifs rise with the increase of genome AT content but vice versa for GC-rich subgroups. Amino acid homopolymer repeats also show the expected quadratic relationship, with higher abundance in species with AT content biased in either direction. Our results show that genome nucleotide composition explains up to half of the variance in the abundance and motif constitution of SSRs.
    • Accession Number:
      0 (DNA, Protozoan)
    • Publication Date:
      Date Created: 20101015 Date Completed: 20110224 Latest Revision: 20110117
    • Publication Date:
      20231215
    • Accession Number:
      10.1093/molbev/msq266
    • Accession Number:
      20943830