Anti-inflammatory role of glycine in reducing rodent postoperative inflammatory ileus.

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  • Additional Information
    • Source:
      Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 9432572 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2982 (Electronic) Linking ISSN: 13501925 NLM ISO Abbreviation: Neurogastroenterol Motil Subsets: MEDLINE
    • Publication Information:
      Original Publication: Osney Mead, Oxford, UK : Blackwell Scientific Publications, c1994-
    • Subject Terms:
      Anti-Inflammatory Agents*/pharmacology ; Anti-Inflammatory Agents*/therapeutic use ; Glycine*/pharmacology ; Glycine*/therapeutic use ; Ileus*/immunology ; Ileus*/pathology; Inflammation/*drug therapy ; Postoperative Complications/*drug therapy; Animals ; Inflammation/immunology ; Male ; Mice ; Mice, Inbred C57BL ; Postoperative Complications/immunology ; Rats ; Receptors, Glycine/metabolism
    • Abstract:
      Background: Inflammatory events within the intestinal muscularis, including macrophage activation and leukocyte recruitment, have been demonstrated to participate in causing postoperative ileus. Recently, glycine has gained attention due to its beneficial immunomodulatory effects in transplantation, shock and sepsis.
      Methods: Muscularis glycine receptors were investigated by immunohistochemistry. Gastrointestinal motility was assessed by in vivo transit distribution histograms with calculated geometric center analysis and jejunal circular smooth muscle contractility in a standard organ bath. The impact of glycine on the muscularis inflammatory responses to surgical manipulation of the intestine were measured by real-time PCR, nitric oxide Griess reaction, prostaglandin ELISA, Luminex and histochemistry.
      Key Results: Glycine-gated chloride channels were immunohistochemically localized to muscularis macrophages and postoperative infiltrating leukocytes. Preoperative glycine treatment significantly improved postoperative gastrointestinal transit and jejunal circular muscle contractility. Preoperative glycine injection significantly reduced the induction of interleukin-6 (IL-6), tumor necrosis factor-α, inducible nitric oxide synthase and intercellular adhesion molecule-1 mRNAs, which was associated with the attenuation in postoperative leukocyte recruitment. Nitric oxide and prostanoid release from the postsurgical inflamed muscularis was diminished by glycine. The secretion of the inflammatory proteins IL-6, monocyte chemotactic protein-1/chemokine ligand 2 and macrophage inflammatory protein-1α/chemokine ligand 3 were also significantly decreased by glycine pretreatment.
      Conclusions & Inferences: The data indicate that preoperative glycine reduces postoperative ileus via the early attenuation of primal inflammatory events within the surgically manipulated gut wall. Therapeutic modulation of resident macrophages by glycine is a potential novel pharmacological target for the prevention of postoperative ileus.
      (© 2010 Blackwell Publishing Ltd.)
    • References:
      Gastroenterology. 2001 Dec;121(6):1354-71. (PMID: 11729115)
      JPEN J Parenter Enteral Nutr. 1981 May-Jun;5(3):215-20. (PMID: 6788969)
      Am J Physiol. 1999 Apr;276(4):G924-32. (PMID: 10198336)
      Transplantation. 1998 Apr 27;65(8):1015-20. (PMID: 9583858)
      Am J Physiol. 1998 Oct;275(4):G862-7. (PMID: 9756519)
      Transpl Int. 2000;13 Suppl 1:S525-7. (PMID: 11112065)
      Br J Surg. 2000 Nov;87(11):1480-93. (PMID: 11091234)
      Nature. 1998 Apr 30;392(6679):933-6. (PMID: 9582075)
      Immunity. 1997 Mar;6(3):315-25. (PMID: 9075932)
      Am J Physiol Gastrointest Liver Physiol. 2001 Jun;280(6):G1076-82. (PMID: 11352799)
      N Engl J Med. 2001 Sep 27;345(13):935-40. (PMID: 11575284)
      Am J Physiol. 1997 Jun;272(6 Pt 1):G1581-6. (PMID: 9227496)
      Nature. 1997 Apr 24;386(6627):855-8. (PMID: 9126747)
      Neurogastroenterol Motil. 2008 Jun;20(6):689-99. (PMID: 18266613)
      Nucleic Acids Res. 1985 Apr 11;13(7):2485-502. (PMID: 2987855)
      Am J Physiol Gastrointest Liver Physiol. 2006 Apr;290(4):G695-703. (PMID: 16537973)
      Am J Physiol Lung Cell Mol Physiol. 2000 Aug;279(2):L390-8. (PMID: 10926563)
      Am J Physiol Gastrointest Liver Physiol. 2002 Oct;283(4):G856-63. (PMID: 12223345)
      Anal Biochem. 2000 Oct 15;285(2):194-204. (PMID: 11017702)
      Gastroenterology. 1999 Aug;117(2):378-87. (PMID: 10419919)
      Dig Dis Sci. 2001 Feb;46(2):230-41. (PMID: 11281168)
      Curr Opin Clin Nutr Metab Care. 2003 Mar;6(2):229-40. (PMID: 12589194)
      Gut. 2003 Apr;52(4):527-34. (PMID: 12631664)
      J Leukoc Biol. 1998 Jun;63(6):683-91. (PMID: 9620660)
      Am J Physiol. 1999 Aug;277(2):G478-86. (PMID: 10444463)
      Ann Surg. 2007 May;245(5):734-44. (PMID: 17457166)
      Anal Biochem. 1982 Oct;126(1):131-8. (PMID: 7181105)
      Ann Surg. 2007 Mar;245(3):355-63. (PMID: 17435541)
      Dis Colon Rectum. 2000 Jan;43(1):61-5. (PMID: 10813125)
      FASEB J. 1999 Mar;13(3):563-71. (PMID: 10064624)
      J Endocrinol Invest. 2008 Aug;31(8):694-9. (PMID: 18852529)
      Br J Surg. 2001 Nov;88(11):1498-500. (PMID: 11683748)
      Gastroenterology. 2000 Feb;118(2):316-27. (PMID: 10648460)
      Surgery. 1999 Sep;126(3):498-509. (PMID: 10486602)
      Ann Plast Surg. 2001 Mar;46(3):320-6. (PMID: 11293527)
      Am J Physiol. 1999 Nov;277(5):L952-9. (PMID: 10564180)
      Cell Mol Life Sci. 1999 Nov 30;56(9-10):843-56. (PMID: 11212343)
      Biochem Biophys Res Commun. 1991 Jan 31;174(2):504-9. (PMID: 1704226)
      Dig Dis Sci. 1990 Jan;35(1):121-32. (PMID: 2403907)
      Langenbecks Arch Chir Suppl Kongressbd. 1998;115:185-90. (PMID: 9931608)
      FASEB J. 2000 Mar;14(3):476-84. (PMID: 10698962)
      Gastroenterology. 2009 May;136(5):1841-2; author reply 1842. (PMID: 19303016)
      Transplantation. 1999 Nov 15;68(9):1244-54. (PMID: 10573059)
      Am J Surg. 2000 Jun;179(6):500-7. (PMID: 11004341)
      J Gastrointest Surg. 1997 Jan-Feb;1(1):61-7; discussion 67-8. (PMID: 9834470)
      Am J Physiol. 1996 Jul;271(1 Pt 1):G97-103. (PMID: 8760112)
      Biochim Biophys Acta. 1992 May 7;1131(1):108-10. (PMID: 1349828)
      Surg Endosc. 2001 Jul;15(7):660-2. (PMID: 11591963)
      Ann Surg. 1998 Nov;228(5):652-63. (PMID: 9833803)
      Neurogastroenterol Motil. 2008 May;20 Suppl 1:81-90. (PMID: 18402645)
      Am J Physiol. 1997 Sep;273(3 Pt 1):G727-34. (PMID: 9316478)
      Surgery. 1993 Sep;114(3):564-70. (PMID: 7690162)
      J Biol Chem. 1989 Sep 25;264(27):16072-82. (PMID: 2789217)
      Cell. 1995 Jan 27;80(2):259-68. (PMID: 7834745)
      J Immunol. 1997 May 1;158(9):4422-9. (PMID: 9127007)
      Transplantation. 1996 Jul 27;62(2):173-8. (PMID: 8755812)
      Gastroenterology. 2009 Feb;136(2):619-29. (PMID: 19014943)
      J Biol Chem. 2000 Jul 28;275(30):23012-9. (PMID: 10816563)
      J Physiol. 2001 Nov 1;536(Pt 3):727-39. (PMID: 11691868)
      Infect Immun. 2001 Sep;69(9):5883-91. (PMID: 11500467)
      Lancet. 1995 Mar 25;345(8952):763-4. (PMID: 7891489)
      Free Radic Biol Med. 2001 Nov 15;31(10):1236-44. (PMID: 11705702)
      Shock. 1999 Jul;12(1):54-62. (PMID: 10468052)
      Gut. 1999 Nov;45(5):713-8. (PMID: 10517907)
      Nat Med. 1998 Nov;4(11):1329-33. (PMID: 9809560)
    • Grant Information:
      R01 DK068610-05 United States DK NIDDK NIH HHS; R01 GM058241 United States GM NIGMS NIH HHS; R01 DK068610 United States DK NIDDK NIH HHS; P50-GM-53789 United States GM NIGMS NIH HHS; R01-DK068610 United States DK NIDDK NIH HHS; P50 GM053789 United States GM NIGMS NIH HHS; DK02488 United States DK NIDDK NIH HHS; R01-GM58241 United States GM NIGMS NIH HHS; R01 GM058241-05 United States GM NIGMS NIH HHS; R01 GM058241-09 United States GM NIGMS NIH HHS
    • Accession Number:
      0 (Anti-Inflammatory Agents)
      0 (Receptors, Glycine)
      TE7660XO1C (Glycine)
    • Publication Date:
      Date Created: 20101014 Date Completed: 20110324 Latest Revision: 20211020
    • Publication Date:
      20240829
    • Accession Number:
      PMC2999652
    • Accession Number:
      10.1111/j.1365-2982.2010.01603.x
    • Accession Number:
      20939853