Molecular characterization of antigen B2 subunit in two genotypes of Echinococcus granulosus from Indian bubaline isolates, its stage specific expression and serological evaluation.

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  • Additional Information
    • Source:
      Publisher: Reidel Country of Publication: Netherlands NLM ID: 0403234 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-4978 (Electronic) Linking ISSN: 03014851 NLM ISO Abbreviation: Mol Biol Rep Subsets: MEDLINE
    • Publication Information:
      Original Publication: Dordrecht, Boston, Reidel.
    • Subject Terms:
      Antigens, Helminth/*genetics ; Echinococcus granulosus/*genetics ; Echinococcus granulosus/*immunology ; Life Cycle Stages/*genetics ; Protein Subunits/*genetics; Animals ; Antigens, Helminth/immunology ; Cattle ; Echinococcus granulosus/growth & development ; Echinococcus granulosus/isolation & purification ; Enzyme-Linked Immunosorbent Assay ; Genotype ; India ; Phylogeny ; Polymorphism, Single-Stranded Conformational ; Protein Subunits/immunology ; Recombinant Proteins/immunology ; Serotyping
    • Abstract:
      Echinococcus granulosus is a parasitic helminth which affects both man and animals. During infection with larval stage of the organism secretory and membrane-bound (S/M) proteins play a meaningful role for evasion of immune system. Antigen B (AgB) is one of them. Present investigation has defined sequence diversity of AgB2 subunit of cattle and buffalo isolates of the organism. A total of 55 isolates were screened by polymerase chain reaction based single stranded conformation polymorphism (PCR-SSCP). Subsequently, six conformers could be detected. Based on predicted amino acid sequences of 90 amino acid residues, three clusters could be deduced. Sequence information of two buffalo isolates was homologous to AgB4 indicating gene switching phenomenon in between closely related isoforms. Numerical value of Tajima's D test proved negative selection pressure. Using artificial neural network (ANN), B cell linear epitope and stretches of agretope were predicted. Three clusters could be defined on the basis of B cell linear epitope. Out of three clusters, two showed more than 50% binding propensity with same MHCII alleles whereas, cluster 3 exhibited binding propensity with other MHCII alleles (DRB1_1501, DRB1_1502). Relative expression of AgB2 was more in active cysts (1.636 ± 0.092) followed by degenerating (0.449 ± 0.037) and calcified (0.255 ± 0.008). This result suggested that relative expression of AgB2 declines with progression of the disease. Using recombinant AgB2 sensitivity, specificity and accuracy of the ELISA test was 96.7, 94.7 and 95.9%, respectively. No cross reactivity was found with common cestode and trematode infected cattle and buffalo because cross reactive antigen was expressed intracellularly. Finally, this was concluded that AgB2 is the suitable immunological marker for detection, diagnosis and progression of the disease.
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    • Accession Number:
      0 (Antigens, Helminth)
      0 (Protein Subunits)
      0 (Recombinant Proteins)
    • Publication Date:
      Date Created: 20100921 Date Completed: 20110523 Latest Revision: 20211020
    • Publication Date:
      20231215
    • Accession Number:
      10.1007/s11033-010-0332-7
    • Accession Number:
      20852940