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Proteome analysis of rat bone marrow mesenchymal stem cell differentiation.
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- Author(s): Çelebi B;Çelebi B; Elçin AE; Elçin YM
- Source:
Journal of proteome research [J Proteome Res] 2010 Oct 01; Vol. 9 (10), pp. 5217-27.
- Publication Type:
Journal Article; Research Support, Non-U.S. Gov't
- Language:
English
- Additional Information
- Source:
Publisher: American Chemical Society Country of Publication: United States NLM ID: 101128775 Publication Model: Print Cited Medium: Internet ISSN: 1535-3907 (Electronic) Linking ISSN: 15353893 NLM ISO Abbreviation: J Proteome Res Subsets: MEDLINE
- Publication Information:
Original Publication: Washington, D.C. : American Chemical Society, c2002-
- Subject Terms:
- Abstract:
Bone marrow multipotent stromal cells (or mesenchymal stem cells; MSCs) have the capacity for renewal and the potential to differentiate in culture into several cell types including osteoblasts, chondrocytes, adipocytes, cardiomyocytes, and neurons. This study was designed to investigate the protein expression profiles of rat bone marrow MSCs during differentiation into adipogenic (by dexamethasone, isobutylmethylxanthine, insulin, and indomethacin), cardiomyogenic (by 5-azacytidine), chondrogenic (by ascorbic acid, insulin-transferrin-selenous acid, and transforming growth factor-β1), and osteogenic (by dexamethasone, β-glycerophosphate, and ascorbic acid) lineages by well-known differentiation inducers. Proteins extracted from differentiated MSCs were separated using two-dimensional gel electrophoresis (2-DE) and protein spots were detected using Sypro Ruby dye. Protein spots that were determined to be up- or down-regulated when the expression of corresponding spots (between weeks 1 and 2, 1 and 3, 1 and 4) showed an increase (≥2-fold) or decrease (≤0.5-fold) were successfully identified by MALDI-TOF-MS. In summary, 23 new proteins were identified either up- or down-regulated during differentiation experiments.
- Accession Number:
0 (Proteome)
7S5I7G3JQL (Dexamethasone)
M801H13NRU (Azacitidine)
XXE1CET956 (Indomethacin)
- Publication Date:
Date Created: 20100805 Date Completed: 20101029 Latest Revision: 20181201
- Publication Date:
20221213
- Accession Number:
10.1021/pr100506u
- Accession Number:
20681633
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