Mycobacterium paratuberculosis monoassociated nude mice as a paratuberculosis model.

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  • Author(s): Hamilton HL;Hamilton HL; Cooley AJ; Adams JL; Czuprynski CJ
  • Source:
    Veterinary pathology [Vet Pathol] 1991 Mar; Vol. 28 (2), pp. 146-55.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Sage Country of Publication: United States NLM ID: 0312020 Publication Model: Print Cited Medium: Print ISSN: 0300-9858 (Print) Linking ISSN: 03009858 NLM ISO Abbreviation: Vet Pathol Subsets: MEDLINE
    • Publication Information:
      Publication: Jan. 2010- : Thousand Oaks, CA : Sage
      Original Publication: 1971- : Basel : New York : Karger.
    • Subject Terms:
      Disease Models, Animal* ; Mice, Inbred BALB C* ; Mice, Nude*; Mycobacterium/*isolation & purification ; Paratuberculosis/*microbiology ; Tumor Necrosis Factor-alpha/*metabolism; Animals ; Feces/microbiology ; Intestines/microbiology ; Liver/microbiology ; Mice ; Paratuberculosis/blood ; Paratuberculosis/pathology
    • Abstract:
      In this study, a paratuberculosis (Johne's disease) model was developed by intragastrically dosing gnotobiotic athymic nude mice with Mycobacterium paratuberculosis. The mice infrequently shed bacilli from their intestinal tracts during the first 4 months after inoculation. Following this time, increasing numbers of M. paratuberculosis (greater than 4.0 log10 bacilli per fecal pellet by 40 weeks) were recovered from the feces of the 12 mice that remained in the isolator. A similar pattern of recovery of M. paratuberculosis was obtained from the ileum, cecum, colon, and liver. Histopathologic lesions and acid-fast bacilli were rare during the first 4 months of infection and then, with time, increased in prevalence and severity. Mice maintained for 7 months or longer exhibited severe granulomatous inflammation and large numbers of acid-fast bacilli in the gastrointestinal tract and liver (up to 10(8) log10 colony forming units per gram wet weight). Five mice maintained for 7 months or more developed clinical signs consistent with those seen in paratuberculosis (weight loss, chronic diarrhea); three of these mice eventually died or became moribund and were euthanatized. M. paratuberculosis monoassociated mice released increased levels of tumor necrosis factor activity into their sera, as compared to uninfected control mice, when they were injected with bacterial lipopolysaccharide. The clinical signs, fecal shedding of M. paratuberculosis, granuloma formation, and progressive bacillary multiplication observed with these mice are consistent with naturally occurring M. paratuberculosis infection of ruminants (Johne's disease). This model will be useful for future studies of immunoregulation and antimicrobial therapy of paratuberculosis.
    • Grant Information:
      R01 AI21343 United States AI NIAID NIH HHS
    • Accession Number:
      0 (Tumor Necrosis Factor-alpha)
    • Publication Date:
      Date Created: 19910301 Date Completed: 19910802 Latest Revision: 20181130
    • Publication Date:
      20250114
    • Accession Number:
      10.1177/030098589102800207
    • Accession Number:
      2063515