The pdh operon is expressed in a subpopulation of stationary-phase bacteria and is important for survival of sugar-starved Streptococcus mutans.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Author(s): Busuioc M;Busuioc M; Buttaro BA; Piggot PJ
  • Source:
    Journal of bacteriology [J Bacteriol] 2010 Sep; Vol. 192 (17), pp. 4395-402. Date of Electronic Publication: 2010 Jun 25.
  • Publication Type:
    Journal Article; Research Support, N.I.H., Extramural
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 2985120R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5530 (Electronic) Linking ISSN: 00219193 NLM ISO Abbreviation: J Bacteriol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, DC : American Society for Microbiology
    • Subject Terms:
      Bacterial Proteins/*metabolism ; Glucose/*metabolism ; Operon/*physiology ; Pyruvate Dehydrogenase Complex/*metabolism ; Streptococcus mutans/*growth & development ; Streptococcus mutans/*physiology; Bacterial Proteins/genetics ; Biofilms/growth & development ; Carbohydrate Metabolism ; Culture Media ; Dental Plaque/microbiology ; Gene Expression Regulation, Bacterial ; Humans ; Operon/genetics ; Pyruvate Dehydrogenase Complex/genetics ; Streptococcus mutans/genetics ; Streptococcus mutans/metabolism
    • Abstract:
      Streptococcus mutans is a facultative member of the oral plaque and is associated with dental caries. It is able to survive long periods of sugar starvation. We show here that inactivation of pdhD, putatively encoding a subunit of the pyruvate dehydrogenase complex, impairs survival of both batch cultures and biofilms. We show that pdhD and the downstream genes pdhA, pdhB, and pdhC form an operon that is predominantly transcribed in stationary phase. Analysis with fluorescent reporters revealed a bimodal expression pattern for the pdh promoter, with less than 1% of stationary-phase populations expressing pdh. When it was first detected, after 1 day of sugar starvation in batch culture, expression was mostly in individual bacteria. At later times, expressing bacteria were often in chains. The lengths of the chains increased with time. We infer that the pdh-expressing subpopulation is able grow and divide and to persist for extended times in stationary phase.
    • References:
      J Bacteriol. 1986 May;166(2):658-65. (PMID: 3009419)
      J Bacteriol. 1992 Jul;174(14):4838-41. (PMID: 1624471)
      Nat Rev Microbiol. 2007 Jan;5(1):48-56. (PMID: 17143318)
      Mol Microbiol. 2006 Aug;61(3):564-72. (PMID: 16879639)
      J Bacteriol. 2002 May;184(10):2780-8. (PMID: 11976308)
      J Bacteriol. 1975 Oct;124(1):55-61. (PMID: 1176435)
      Biochim Biophys Acta. 1998 Jun 29;1385(2):367-72. (PMID: 9655937)
      Appl Environ Microbiol. 2004 Oct;70(10):6181-7. (PMID: 15466565)
      Microbiol Rev. 1986 Dec;50(4):353-80. (PMID: 3540569)
      Infect Immun. 1985 Sep;49(3):674-8. (PMID: 4030096)
      Soc Appl Bacteriol Symp Ser. 1997;26:12S-19S. (PMID: 9436313)
      Adv Dent Res. 1997 Apr;11(1):75-80. (PMID: 9524445)
      Science. 1964 Nov 6;146(3645):775-7. (PMID: 14197565)
      J Bacteriol. 2009 Dec;191(23):7315-22. (PMID: 19801415)
      Biochem J. 1980 Jul 1;189(1):161-72. (PMID: 7458900)
      Infect Immun. 1995 Jan;63(1):9-20. (PMID: 7806389)
      Curr Opin Microbiol. 2009 Dec;12(6):667-73. (PMID: 19854099)
      Annu Rev Microbiol. 2008;62:193-210. (PMID: 18537474)
      FEMS Microbiol Lett. 2009 Oct;299(2):261-6. (PMID: 19735463)
      J Bacteriol. 1985 Nov;164(2):782-96. (PMID: 2997130)
      Science. 2003 Jul 25;301(5632):510-3. (PMID: 12817086)
      Gene. 1996 Oct 24;177(1-2):137-47. (PMID: 8921859)
      Caries Res. 1970;4(4):305-20. (PMID: 5276567)
      Infect Immun. 1981 Jun;32(3):1295-7. (PMID: 7251168)
      FEMS Microbiol Lett. 2008 Dec;289(1):13-9. (PMID: 19054088)
      FEMS Microbiol Lett. 2005 Jun 15;247(2):171-6. (PMID: 15925458)
      Infect Immun. 1975 Apr;11(4):649-55. (PMID: 1091546)
      Soc Appl Bacteriol Symp Ser. 1997;26:80S-88S. (PMID: 9436320)
    • Grant Information:
      R01 DE014604 United States DE NIDCR NIH HHS; DE014604 United States DE NIDCR NIH HHS
    • Accession Number:
      0 (Bacterial Proteins)
      0 (Culture Media)
      0 (Pyruvate Dehydrogenase Complex)
      IY9XDZ35W2 (Glucose)
    • Publication Date:
      Date Created: 20100629 Date Completed: 20100913 Latest Revision: 20211020
    • Publication Date:
      20240829
    • Accession Number:
      PMC2937364
    • Accession Number:
      10.1128/JB.00574-10
    • Accession Number:
      20581205