Developmental changes in cellular and extracellular structural macromolecules in the secondary palate and in the nasal cavity of the mouse.

Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 9504563 Publication Model: Print Cited Medium: Internet ISSN: 1600-0722 (Electronic) Linking ISSN: 09098836 NLM ISO Abbreviation: Eur J Oral Sci Subsets: MEDLINE

Publication: Chichester : Wiley-Blackwell
Original Publication: Copenhagen : Munksgaard, c1995-

Cytoskeletal Proteins/*analysis ; Extracellular Matrix Proteins/*analysis ; Nasal Cavity/*embryology ; Palate/*embryology; Animals ; Apoptosis/physiology ; Cell Adhesion/physiology ; Chondroitin Sulfates/analysis ; Epithelium/embryology ; Gestational Age ; Heparitin Sulfate/analysis ; Hepatocyte Nuclear Factor 3-alpha/analysis ; Keratin-14/analysis ; Keratin-15/analysis ; Keratin-6/analysis ; Keratin-8/analysis ; Keratins/analysis ; Lamin Type A/analysis ; Lewis X Antigen/analysis ; Macromolecular Substances ; Mice ; Myosin Heavy Chains/analysis ; Nasal Cavity/cytology ; Nonmuscle Myosin Type IIA/analysis ; Palate/cytology ; Palate/innervation ; Protein Serine-Threonine Kinases/analysis ; Receptor, Transforming Growth Factor-beta Type I ; Receptors, Transforming Growth Factor beta/analysis ; Signal Transduction/physiology ; Transforming Growth Factor beta3/analysis ; Up-Regulation ; Versicans/analysis ; Vomer/cytology ; Vomer/embryology

The aim of this study was to analyse the hitherto largely unknown expression patterns of some specific cellular and extracellular molecules during palate and nasal cavity development. We showed that epithelia of the developing palate and the vomerine epithelium express similar sets of structural proteins. With the exception of keratin 15, which becomes barely detectable in the elevated palatal shelves, nearly all of these proteins become upregulated at the presumptive areas of fusion and in the adhering epithelia of the palate and nasal septum. In vivo and in vitro analyses indicated that reduction in the amount of keratin 15 protein is independent of Tgfbeta-Alk5 signalling. Foxa1 expression also highlighted the regionalization of the palatal and nasal epithelia. Owing to the lack of reliable markers of the palatal periderm, the fate of peridermal cells has been controversial. We identified LewisX/stage-specific embryonic antigen-1 as a specific peridermal marker, and showed that numerous peridermal cells remain trapped in the medial epithelial seam (MES). The fate of these cells is probably apoptosis together with the rest of the MES cells, as we provided strong evidence for this event. Heparan sulphate, chondroitin-6-sulphate, and versican displayed dynamically changing distribution patterns. The hitherto-unknown innervation pattern of the developing palate was revealed. These findings may be of value for unravelling the pathogenesis of palatal clefting.

0 (Cytoskeletal Proteins)
0 (Extracellular Matrix Proteins)
0 (Foxa1 protein, mouse)
0 (Hepatocyte Nuclear Factor 3-alpha)
0 (Keratin-14)
0 (Keratin-15)
0 (Keratin-6)
0 (Keratin-8)
0 (Krt14 protein, mouse)
0 (Krt15 protein, mouse)
0 (Krt17 protein, mouse)
0 (Krt8 protein, mouse)
0 (Lamin Type A)
0 (Lewis X Antigen)
0 (Macromolecular Substances)
0 (Receptors, Transforming Growth Factor beta)
0 (Tgfb3 protein, mouse)
0 (Transforming Growth Factor beta3)
0 (Vcan protein, mouse)
126968-45-4 (Versicans)
68238-35-7 (Keratins)
9007-28-7 (Chondroitin Sulfates)
9050-30-0 (Heparitin Sulfate)
EC 2.7.11.1 (Protein Serine-Threonine Kinases)
EC 2.7.11.30 (Receptor, Transforming Growth Factor-beta Type I)
EC 2.7.11.30 (Tgfbr1 protein, mouse)
EC 3.6.1.- (Nonmuscle Myosin Type IIA)
EC 3.6.4.1 (Myosin Heavy Chains)

Date Created: 20100625 Date Completed: 20100928 Latest Revision: 20211203

20221213

10.1111/j.1600-0722.2010.00732.x

20572855