Underestimation of vancomycin and teicoplanin MICs by broth microdilution leads to underdetection of glycopeptide-intermediate isolates of Staphylococcus aureus.

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  • Author(s): Vaudaux P;Vaudaux P; Huggler E; Bernard L; Ferry T; Renzoni A; Lew DP
  • Source:
    Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2010 Sep; Vol. 54 (9), pp. 3861-70. Date of Electronic Publication: 2010 Jun 14.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 0315061 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-6596 (Electronic) Linking ISSN: 00664804 NLM ISO Abbreviation: Antimicrob Agents Chemother Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, American Society for Microbiology
    • Subject Terms:
      Anti-Bacterial Agents/*pharmacology ; Glycopeptides/*pharmacology ; Microbial Sensitivity Tests/*methods ; Staphylococcus aureus/*drug effects ; Teicoplanin/*pharmacology ; Vancomycin/*pharmacology
    • Abstract:
      Broth microdilution was compared with tube macrodilution and a simplified population analysis agar method for evaluating vancomycin and teicoplanin MICs and detecting glycopeptide-intermediate isolates of Staphylococcus aureus. Modal vancomycin and teicoplanin MICs recorded by tube macrodilution and the agar plate assay, which both used inocula of 10(6) CFU, were significantly higher (2 microg/ml) against a panel of borderline glycopeptide-susceptible and glycopeptide-intermediate methicillin-resistant S. aureus (MRSA) bloodstream isolates compared to broth microdilution (1 microg/ml). Vancomycin and teicoplanin MIC distributions by tube macrodilution and agar testing were also markedly different from those evaluated by broth microdilution. The 20-fold-lower inoculum size used for broth microdilution compared to macrodilution and agar MIC assays explained in part, but not entirely, the systematic trend toward lower vancomycin and teicoplanin MICs by microdilution compared to other methods. Broth microdilution assay led to underdetection of the vancomycin-intermediate S. aureus (VISA) phenotype, yielding only three VISA isolates, for which vancomycin MICs were 4 microg/ml compared to 8 and 19 VISA isolates detected by macrodilution and agar testing, respectively. While macrodilution and agar testing detected 7 and 22 isolates with elevated teicoplanin MICs (8 microg/ml), respectively, broth microdilution failed to detect such isolates. Detection rates of isolates with elevated vancomycin and teicoplanin MICs by macrodilution and agar testing assays were higher at 48 h than at 24 h. In conclusion, the sensitivity of broth microdilution MIC testing is questionable for reliable detection and epidemiological surveys of glycopeptide-intermediate resistance in S. aureus isolates.
    • References:
      J Antimicrob Chemother. 1997 Jul;40(1):135-6. (PMID: 9249217)
      Clin Ther. 2006 Aug;28(8):1208-1216. (PMID: 16982298)
      Clin Infect Dis. 2007 Jun 15;44(12):1536-42. (PMID: 17516395)
      Antimicrob Agents Chemother. 2010 Feb;54(2):945-9. (PMID: 19949062)
      Antimicrob Agents Chemother. 2008 Sep;52(9):3315-20. (PMID: 18591266)
      J Antimicrob Chemother. 2001 Apr;47(4):399-403. (PMID: 11266410)
      Int J Antimicrob Agents. 2009 Jun;33(6):564-8. (PMID: 19233622)
      Clin Microbiol Infect. 2008 Feb;14(2):101-4. (PMID: 18093235)
      N Engl J Med. 2003 Apr 3;348(14):1342-7. (PMID: 12672861)
      J Clin Microbiol. 2009 Jul;47(7):2013-7. (PMID: 19420170)
      J Clin Microbiol. 2001 Jul;39(7):2439-44. (PMID: 11427551)
      J Clin Microbiol. 2004 Jun;42(6):2398-402. (PMID: 15184410)
      Antimicrob Agents Chemother. 2003 Oct;47(10):3040-5. (PMID: 14506006)
      Clin Microbiol Rev. 2010 Jan;23(1):99-139. (PMID: 20065327)
      J Clin Microbiol. 2006 Nov;44(11):3883-6. (PMID: 16957043)
      J Clin Microbiol. 2003 Apr;41(4):1687-93. (PMID: 12682161)
      Antimicrob Agents Chemother. 2003 Apr;47(4):1262-6. (PMID: 12654656)
      Ann Clin Microbiol Antimicrob. 2007 Oct 30;6:13. (PMID: 17967199)
      J Clin Microbiol. 2007 Feb;45(2):329-32. (PMID: 17108069)
      Diagn Microbiol Infect Dis. 1998 Aug;31(4):563-71. (PMID: 9764397)
      J Antimicrob Chemother. 2005 Dec;56(6):1000-18. (PMID: 16293678)
      Antimicrob Agents Chemother. 2007 Jul;51(7):2582-6. (PMID: 17452488)
      Clin Microbiol Rev. 2007 Jul;20(3):391-408, table of contents. (PMID: 17630331)
      Antimicrob Agents Chemother. 2009 Oct;53(10):4127-32. (PMID: 19635961)
      Clin Infect Dis. 2006 Jan 1;42 Suppl 1:S13-24. (PMID: 16323115)
      Antimicrob Agents Chemother. 1999 Aug;43(8):1914-8. (PMID: 10428912)
      Arch Intern Med. 2006 Oct 23;166(19):2138-44. (PMID: 17060545)
      Clin Infect Dis. 2007 May 1;44(9):1208-15. (PMID: 17407040)
      J Antimicrob Chemother. 2007 Oct;60(4):788-94. (PMID: 17623693)
      Antimicrob Agents Chemother. 2008 Dec;52(12):4528. (PMID: 18838599)
      Antimicrob Agents Chemother. 2005 Sep;49(9):3982-3. (PMID: 16127089)
      Antimicrob Agents Chemother. 2008 Oct;52(10):3755-62. (PMID: 18644967)
      J Clin Microbiol. 2007 May;45(5):1511-4. (PMID: 17344363)
      Clin Infect Dis. 2008 Jan 15;46(2):193-200. (PMID: 18171250)
      Clin Infect Dis. 2004 Feb 1;38(3):448-51. (PMID: 14727222)
      Eur J Clin Microbiol Infect Dis. 2010 Feb;29(2):171-80. (PMID: 19946789)
      J Infect Dis. 2009 Nov 1;200(9):1355-66. (PMID: 19811099)
      Antimicrob Agents Chemother. 2006 Sep;50(9):3039-47. (PMID: 16940100)
      J Microbiol Methods. 2004 May;57(2):231-9. (PMID: 15063063)
      J Clin Microbiol. 2008 Sep;46(9):3042-7. (PMID: 18596146)
      Antimicrob Agents Chemother. 2000 Feb;44(2):294-303. (PMID: 10639353)
      Clin Infect Dis. 2008 Mar 1;46(5):668-74. (PMID: 18257700)
      Clin Infect Dis. 2007 Jun 15;44(12):1543-8. (PMID: 17516396)
      BMC Microbiol. 2008 Feb 27;8:39. (PMID: 18304359)
      Antimicrob Agents Chemother. 2009 Sep;53(9):3642-9. (PMID: 19546358)
      Lancet. 1997 Dec 6;350(9092):1670-3. (PMID: 9400512)
      Trends Microbiol. 2010 Feb;18(2):55-6. (PMID: 20005114)
      J Antimicrob Chemother. 2008 Jan;61(1):85-90. (PMID: 18042628)
      J Clin Microbiol. 2007 Oct;45(10):3263-9. (PMID: 17687008)
      Lancet Infect Dis. 2001 Oct;1(3):147-55. (PMID: 11871491)
      J Clin Microbiol. 1998 Apr;36(4):1020-7. (PMID: 9542929)
      Int J Antimicrob Agents. 2008 Apr;31 Suppl 2:1-9. (PMID: 18485997)
      Int J Antimicrob Agents. 2007 Jul;30(1):1-3. (PMID: 17507206)
      Annu Rev Microbiol. 2002;56:657-75. (PMID: 12142482)
    • Accession Number:
      0 (Anti-Bacterial Agents)
      0 (Glycopeptides)
      61036-62-2 (Teicoplanin)
      6Q205EH1VU (Vancomycin)
    • Publication Date:
      Date Created: 20100616 Date Completed: 20101201 Latest Revision: 20211020
    • Publication Date:
      20231215
    • Accession Number:
      PMC2934981
    • Accession Number:
      10.1128/AAC.00269-10
    • Accession Number:
      20547791