[Effects of anion channel blockers NPPB and DIDS on tobacco pollen tube growth and its mitochondria state].

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  • Additional Information
    • Source:
      Publisher: Rossiiskaia Akademiia Nauk Country of Publication: Russia (Federation) NLM ID: 0417363 Publication Model: Print Cited Medium: Print ISSN: 0041-3771 (Print) Linking ISSN: 00413771 NLM ISO Abbreviation: Tsitologiia Subsets: MEDLINE
    • Publication Information:
      Publication: Moskva : Rossiiskaia Akademiia Nauk
      Original Publication: Moskva, Akademiia nauk SSSR.
    • Subject Terms:
      4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid/*pharmacology ; Ion Channels/*antagonists & inhibitors ; Nitrobenzoates/*pharmacology ; Pollen Tube/*drug effects ; Nicotiana/*drug effects; Membrane Potential, Mitochondrial/drug effects ; Mitochondria/drug effects ; Pollen Tube/growth & development ; Nicotiana/growth & development
    • Abstract:
      Influence of anion channel blockers NPPB and DIDS on pollen tube growth and its mitochondria functioning was studied by means of fluorescence microscopy and flow cytometry. NPPB (40 microM) blocked pollen tube growth completely, but did not change its diameter. DIDS (20-80 microM) caused pollen tube swelling and bursting, suggesting that DIDS-sensitive channels take part in the regulation of pollen tube osmotic balance. The osmotic effect of low DIDS concentration (20 (Mkappa)M) was not accompanied by changes in the tube growth rate. The mapping of membrane potential on the pollen tube plasmalemma using Di-4-ANEPPS revealed the involvement of NPPB-sensitive but not DIDS-sensitive anion channels in the maintenance of the longitudinal membrane potential gradient along the tube surface. The study of isolated pollen mitochondria showed that DIDS increased their capacity to take up potential-dependent dye DiOC5(3), i. e. caused hyperpolarization of mitochondrial membranes. At the same time DIDS influenced on intramitochondrial ROS content and excretion of ROS from mitochondria. Thus, NPPB and DIDS differently influenced on transmembrane potential distribution along pollen tube plasmalemma, on its osmotic balance, and on mitochondria functioning. This set of data suggests that pollen tube growth is dependent on activity of anion channels that differ in localization and functions.
    • Accession Number:
      0 (Ion Channels)
      0 (Nitrobenzoates)
      3A35O9G3YZ (5-nitro-2-(3-phenylpropylamino)benzoic acid)
      Q1O6DSW23R (4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid)
    • Publication Date:
      Date Created: 20100615 Date Completed: 20100824 Latest Revision: 20231213
    • Publication Date:
      20231215
    • Accession Number:
      20540345