Upregulation of ANP and NPR-C mRNA in the kidney and heart of eNOS knockout mice.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Author(s): Yuan K;Yuan K; Kim SY; Oh YB; Yu J; Shah A; Park BH; Kim SH
  • Source:
    Peptides [Peptides] 2010 Jul; Vol. 31 (7), pp. 1319-25. Date of Electronic Publication: 2010 Apr 18.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Elsevier Science Inc Country of Publication: United States NLM ID: 8008690 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-5169 (Electronic) Linking ISSN: 01969781 NLM ISO Abbreviation: Peptides Subsets: MEDLINE
    • Publication Information:
      Publication: New York, NY : Elsevier Science Inc.
      Original Publication: Fayetteville, N. Y., Ankho International.
    • Subject Terms:
      Up-Regulation*; Atrial Natriuretic Factor/*metabolism ; Kidney/*metabolism ; Myocardium/*metabolism ; Nitric Oxide Synthase Type III/*genetics ; Receptors, Atrial Natriuretic Factor/*metabolism; Animals ; Atrial Natriuretic Factor/genetics ; Male ; Mice ; Mice, Knockout ; Nitric Oxide Synthase Type III/metabolism ; RNA, Messenger/metabolism ; Receptors, Atrial Natriuretic Factor/genetics ; Transcription Factors/genetics ; Transcription Factors/metabolism
    • Abstract:
      Objectives: The aim of the present studywas to examine the question of whether the atrial natriuretic peptide (ANP) system is altered by endothelial nitric-oxide synthase (eNOS).
      Methods: Male eNOS-deficient mice (eNOS-/-) and wild type control mice (eNOS+/+, C57B1/6J) were used. Blood pressure was measured in anesthetized mice by tail cuff plethysmography and renal function was measured. Expression of ANP, natriuretic peptide receptor (NPR)-A, NPR-C, and tonicity-responsive enhancer binding protein (TonEBP) mRNA was determined by real-time PCR. Localization of (125)I-ANP binding sites was measured using in vitro autoradiography.
      Results: In eNOS-/- mice, systolic blood pressure increased and left ventricular hypertrophy was observed. Urine volume and osmolarity did not change. Expression of ANP markedly increased in the heart and kidney of eNOS-/- mice. Expression of NPR-A and NPR-C increased in the heart and tended to increase in the kidney of eNOS-/- mice. In the renal medulla in particular, increased expression of NPR-C was more prominent. Expression of TonEBP mRNA was markedly decreased in the renal medulla, but not in the renal cortex. Maximum binding capacity (B(max)) of ANP and C-ANP increased in the renal medulla in eNOS-/- mice.
      Conclusion: These results suggest that the eNOS-NO system may be partly involved in regulation of ANP, NPR-A, -C, and TonEBP mRNA expression in the kidney.
      (Copyright 2010 Elsevier Inc. All rights reserved.)
    • Accession Number:
      0 (Nfat5 protein, mouse)
      0 (RNA, Messenger)
      0 (Transcription Factors)
      85637-73-6 (Atrial Natriuretic Factor)
      EC 1.14.13.39 (Nitric Oxide Synthase Type III)
      EC 4.6.1.2 (Receptors, Atrial Natriuretic Factor)
      EC 4.6.1.2 (atrial natriuretic factor receptor C)
    • Publication Date:
      Date Created: 20100421 Date Completed: 20100914 Latest Revision: 20100531
    • Publication Date:
      20221213
    • Accession Number:
      10.1016/j.peptides.2010.04.008
    • Accession Number:
      20403400