In situ hybridization banding of human chromosomes with Alu-PCR products: a simultaneous karyotype for gene mapping studies.

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  • Author(s): Baldini A;Baldini A; Ward DC
  • Source:
    Genomics [Genomics] 1991 Apr; Vol. 9 (4), pp. 770-4.
  • Publication Type:
    Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Academic Press Country of Publication: United States NLM ID: 8800135 Publication Model: Print Cited Medium: Print ISSN: 0888-7543 (Print) Linking ISSN: 08887543 NLM ISO Abbreviation: Genomics Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Diego : Academic Press, [c1987-
    • Subject Terms:
      Chromosome Mapping* ; Repetitive Sequences, Nucleic Acid*; Chromosome Banding/*methods; DNA ; DNA Probes ; Humans ; Karyotyping ; Nucleic Acid Hybridization ; Polymerase Chain Reaction
    • Abstract:
      Polymerase chain reaction products generated from a single Alu primer and human genomic DNA produce a distinct and highly reproducible R-banding pattern when hybridized to metaphase chromosome spreads. Individual chromosomes can be readily identified and karyotyped. Compared to conventional fluorescence banding on heat-denatured chromosomes, the in situ hybridization banding (ISHB) shows high contrast and definition. We demonstrate that this banding method can be employed effectively in double-labeling experiments for the rapid and simultaneous assignment of probes to specific chromosomal bands. Since virtually any fluorochrome can be used to delineate chromosomal bands, ISHB should provide added flexibility for multicolor mapping strategies.
    • Grant Information:
      HG00246 United States HG NHGRI NIH HHS; HG00272 United States HG NHGRI NIH HHS
    • Accession Number:
      0 (DNA Probes)
      9007-49-2 (DNA)
    • Publication Date:
      Date Created: 19910401 Date Completed: 19910702 Latest Revision: 20190903
    • Publication Date:
      20221213
    • Accession Number:
      10.1016/0888-7543(91)90374-n
    • Accession Number:
      2037303