Differential expression of the CCN family member WISP-1, WISP-2 and WISP-3 in human colorectal cancer and the prognostic implications.

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  • Additional Information
    • Source:
      Publisher: D.A. Spandidos Country of Publication: Greece NLM ID: 9306042 Publication Model: Print Cited Medium: Internet ISSN: 1791-2423 (Electronic) Linking ISSN: 10196439 NLM ISO Abbreviation: Int J Oncol Subsets: MEDLINE
    • Publication Information:
      Publication: <2003->: Athens, Greece : D.A. Spandidos
      Original Publication: Athens, Greece : Lychnia,
    • Subject Terms:
      Gene Expression Profiling* ; Gene Expression Regulation, Neoplastic*; Colorectal Neoplasms/*diagnosis ; Colorectal Neoplasms/*metabolism ; Insulin-Like Growth Factor Binding Proteins/*biosynthesis ; Intercellular Signaling Peptides and Proteins/*biosynthesis ; Proto-Oncogene Proteins/*biosynthesis ; Transcription Factors/*biosynthesis; CCN Intercellular Signaling Proteins ; Cell Differentiation ; Cell Line, Tumor ; DNA Primers/genetics ; DNA, Complementary/metabolism ; Humans ; Immunohistochemistry/methods ; Intracellular Signaling Peptides and Proteins ; Models, Biological ; Prognosis ; Repressor Proteins
    • Abstract:
      The WISPs (Wnt-inducted secreted proteins, WISP-1, WISP-2 and WISP-3) are part of the CCN family. These molecules are known to play a diverse role in cells but their role in cancer cells remains controversial. We analysed the expression of the three WISP molecules at the mRNA and protein levels in a cohort of 94 human colorectal tumours and 80 normal colorectal tissues and correlated the results with the pathological features and clinical outcome of the patients. WISP-1 transcripts were found at higher levels in the tumour samples than in the normal tissue (p=0.0015); higher in patients with Dukes stage B and C compared to Dukes A (p=0.017 and p=0.024, respectively); higher in patients with moderately and poorly differentiated cancers compared to the well differentiated cancers (p=0.020 and p=0.076, respectively and p=0.0035 when combined); higher in node positive tumours compared with the node negative (p=0.11) and in the patients with higher TNM staging (TNM 2, 3 and 4 compared to TNM 1 p=0.037). WISP-2 showed the opposite pattern with lower levels of expression in cancer cells compared to normal (p=0.082). Although no significant differences were found within the cancer group when indices of a more aggressive tumour were compared to the normal tissue a significant reduction in expression was found (Dukes C p=0.044, poorly differentiated p=0.019, TNM 3 p=0.020 and node positive disease p=0.048). WISP-3 transcript levels showed no significant differences between groups. WISPs may play important but contrasting roles in colorectal cancer with WISP-1 appearing to act as a factor stimulating aggressiveness, WISP-2 as a tumour suppressor and WISP-3 having no definable beneficial or detrimental role.
    • Accession Number:
      0 (CCN Intercellular Signaling Proteins)
      0 (CCN4 protein, human)
      0 (CCN5 protein, human)
      0 (CCN6 protein, human)
      0 (DNA Primers)
      0 (DNA, Complementary)
      0 (Insulin-Like Growth Factor Binding Proteins)
      0 (Intercellular Signaling Peptides and Proteins)
      0 (Intracellular Signaling Peptides and Proteins)
      0 (Proto-Oncogene Proteins)
      0 (Repressor Proteins)
      0 (Transcription Factors)
    • Publication Date:
      Date Created: 20100408 Date Completed: 20101122 Latest Revision: 20190816
    • Publication Date:
      20240829
    • Accession Number:
      10.3892/ijo_00000595
    • Accession Number:
      20372786