Proteomic analysis of Medicago truncatula root plastids.

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  • Additional Information
    • Source:
      Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101092707 Publication Model: Print Cited Medium: Internet ISSN: 1615-9861 (Electronic) Linking ISSN: 16159853 NLM ISO Abbreviation: Proteomics Subsets: MEDLINE
    • Publication Information:
      Original Publication: Weinheim, Germany : Wiley-VCH,
    • Subject Terms:
      Medicago truncatula/*metabolism ; Plant Proteins/*metabolism ; Plant Roots/*metabolism ; Plastids/*metabolism ; Proteomics/*methods; Gene Expression Regulation, Plant/physiology ; Medicago truncatula/physiology ; Plant Roots/physiology ; Plastids/physiology ; Tandem Mass Spectrometry
    • Abstract:
      Despite the recognized importance of non-photosynthetic plastids in a wide array of plant processes, the root plastid proteome of soil-grown plants still remains to be explored. In this study, we used a protocol allowing the isolation of Medicago truncatula root plastids with sufficient protein recovery and purity for their subsequent in-depth analysis by nanoscale capillary LC-MS/MS. Besides providing the first picture of a root plastid proteome, the results obtained highlighted the identification of 266 protein candidates whose functional distribution mainly resembled that of wheat endosperm amyloplasts and tobacco proplastids together with displaying major differences to those reported for chloroplasts. Most of the identified proteins have a role in nucleic acid-related processes (16%), carbohydrate (15%) and nitrogen/sulphur (12%) metabolisms together with stress response mechanisms (10%). It is noteworthy that BLAST searches performed against the proteins reported in different plastidomes allowed detecting 30 putative root plastid proteins for which homologues were previously unsuspected as plastid-located, most of them displaying a common putative role in participating in the plant cell responses against abiotic and/or biotic stresses. Taken together, the data obtained provide new insights into the functioning of root plastids and reinforce the emerging idea for an important role of these organelles in sustaining plant defence reactions.
    • Accession Number:
      0 (Plant Proteins)
    • Publication Date:
      Date Created: 20100326 Date Completed: 20100907 Latest Revision: 20100607
    • Publication Date:
      20231215
    • Accession Number:
      10.1002/pmic.200900345
    • Accession Number:
      20336678