[A preliminary study on role of acid sphingomyelinase in receptor clustering induced by 50-Hz magnetic fields].

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  • Author(s): Li XW;Li XW; Lu DQ; Jiang H; Sun WJ
  • Source:
    Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases [Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi] 2009 Sep; Vol. 27 (9), pp. 516-9.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    Chinese
  • Additional Information
    • Source:
      Publisher: Tianjin shi lao dong wei sheng yan jiu suo ; Tianjin shi lao dong wei sheng huan jing yi xue hui Country of Publication: China NLM ID: 8410840 Publication Model: Print Cited Medium: Print ISSN: 1001-9391 (Print) Linking ISSN: 10019391 NLM ISO Abbreviation: Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi Subsets: MEDLINE
    • Publication Information:
      Original Publication: [Tientsin] : Tianjin shi lao dong wei sheng yan jiu suo ; Tianjin shi lao dong wei sheng huan jing yi xue hui, [1983-
    • Subject Terms:
      Epithelial Cells/*metabolism ; ErbB Receptors/*metabolism ; Magnetic Fields/*adverse effects ; Sphingomyelin Phosphodiesterase/*physiology; Amnion/cytology ; Cell Line ; Cell Membrane/metabolism ; Cell Membrane/radiation effects ; Ceramides/metabolism ; Epithelial Cells/radiation effects ; Humans ; Sphingomyelin Phosphodiesterase/metabolism
    • Abstract:
      Objective: To investigate the relationship among a 50-Hz MF-induced epidermal growth factor receptor (EGFR) clustering, acid sphingomyelinase (A-SMase) and ceramide (CER), and to explore the possible mechanism of receptor clustering.
      Methods: Human amnion (FL) cells were exposed to a 50-Hz sinusoidal magnetic field at 0.4 mT for 15 min with or without imipramine, a specific inhibitor of A-SMase and ceramide pretreatment. EGF treatment served as the positive control and DMSO treatment served as the solvent control. The EGFR was labeled with polyclonal anti-EGFR antibody and the clustering of EGFR was analyzed using immunofluorescence and confocal microscopy. The percentage of cells with EGFR clustering was counted and compared.
      Results: Both EGF treatment and 50-Hz MF exposure could induce EGFR clustering. However, the effect could be eliminated by imipramine pretreatment for 4 hours. When FL cells were incubated with ceramide following the imipramine pretreatment for 30 min, EGFR clustering induced by 50-Hz MF exposure could be recovered.
      Conclusion: EGFR clustering induced by 50-Hz MF depends on A-SMase activity, and ceramide, as the hydrolyzate from A-SMase might participate in the process of EGFR clustering.
    • Accession Number:
      0 (Ceramides)
      EC 2.7.10.1 (ErbB Receptors)
      EC 3.1.4.12 (Sphingomyelin Phosphodiesterase)
    • Publication Date:
      Date Created: 20100209 Date Completed: 20120719 Latest Revision: 20181201
    • Publication Date:
      20231215
    • Accession Number:
      20137294