Neuronal differentiation of NG108-15 cells has impact on nitric oxide- and membrane (natriuretic peptide receptor-A) cyclic GMP-generating proteins.

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  • Author(s): Müller D;Müller D; Greenland KJ; Speth RC; Middendorff R
  • Source:
    Molecular and cellular endocrinology [Mol Cell Endocrinol] 2010 May 14; Vol. 320 (1-2), pp. 118-27. Date of Electronic Publication: 2010 Jan 25.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: North Holland Publishing Country of Publication: Ireland NLM ID: 7500844 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-8057 (Electronic) Linking ISSN: 03037207 NLM ISO Abbreviation: Mol Cell Endocrinol Subsets: MEDLINE
    • Publication Information:
      Publication: Limerick : North Holland Publishing
      Original Publication: Amsterdam, North-Holland.
    • Subject Terms:
      Cell Differentiation*; Cell Membrane/*metabolism ; Cyclic GMP/*biosynthesis ; Neurons/*cytology ; Nitric Oxide/*metabolism ; Receptors, Atrial Natriuretic Factor/*metabolism; Animals ; Biomarkers/metabolism ; Cell Line ; Cell Membrane/enzymology ; Guanylate Cyclase/metabolism ; Neurons/enzymology ; Nitric Oxide Synthase Type I/metabolism ; Protein Phosphatase 2/metabolism ; Protein Subunits/metabolism ; Rats ; Receptors, Cytoplasmic and Nuclear/metabolism ; Signal Transduction ; Soluble Guanylyl Cyclase ; Up-Regulation
    • Abstract:
      Cyclic GMP (cGMP), produced in response to either nitric oxide (NO) or certain peptides, controls important neuronal functions. NG108-15 cells were used to characterize the expression of NO- and cGMP-generating proteins and to identify potential alterations associated with neuronal differentiation (neurite outgrowth). We find that these cells contain exclusively neuronal NO synthase (nNOS) isoforms as well as both NO- (soluble guanylyl cyclase, sGC) and natriuretic peptide- (natriuretic peptide receptor-A, NPR-A) responsive cGMP-producing enzymes. The sGC beta(1) subunit (unlike protein phosphatase 2A subunits) is highly membrane-associated. Membrane concentrations of NPR-A and nNOS, but not sGC beta(1) protein are up-regulated with neuronal differentiation. Intriguingly, the rate of hormone-induced cGMP production by NPR-A is significantly diminished in differentiated cells. These findings support roles for NPR-A, the common receptor of atrial (ANP) and B-type (BNP) natriuretic peptide in mature neurons and provide evidence for pronounced changes in neuronal submembrane cGMP signalling during neuronal differentiation.
      ((c) 2010 Elsevier Ireland Ltd. All rights reserved.)
    • Accession Number:
      0 (Biomarkers)
      0 (Protein Subunits)
      0 (Receptors, Cytoplasmic and Nuclear)
      31C4KY9ESH (Nitric Oxide)
      EC 1.14.13.39 (Nitric Oxide Synthase Type I)
      EC 3.1.3.16 (Protein Phosphatase 2)
      EC 4.6.1.2 (Guanylate Cyclase)
      EC 4.6.1.2 (Receptors, Atrial Natriuretic Factor)
      EC 4.6.1.2 (Soluble Guanylyl Cyclase)
      EC 4.6.1.2 (atrial natriuretic factor receptor A)
      H2D2X058MU (Cyclic GMP)
    • Publication Date:
      Date Created: 20100126 Date Completed: 20100608 Latest Revision: 20161125
    • Publication Date:
      20221213
    • Accession Number:
      10.1016/j.mce.2010.01.022
    • Accession Number:
      20097258