Asymmetric cell division of rice zygotes located in embryo sac and produced by in vitro fertilization.

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  • Author(s): Sato A;Sato A; Toyooka K; Okamoto T
  • Source:
    Sexual plant reproduction [Sex Plant Reprod] 2010 Sep; Vol. 23 (3), pp. 211-7. Date of Electronic Publication: 2010 Jan 07.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Springer International Country of Publication: Germany NLM ID: 100955730 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-2145 (Electronic) Linking ISSN: 09340882 NLM ISO Abbreviation: Sex Plant Reprod Subsets: MEDLINE
    • Publication Information:
      Original Publication: Heidelberg : Springer International, 1988-
    • Subject Terms:
      Cell Division*; Botany/*methods ; Oryza/*cytology ; Oryza/*embryology ; Seeds/*cytology
    • Abstract:
      In angiosperms, a zygote generally divides into an asymmetric two-celled embryo consisting of an apical and a basal cell. This unequal division of the zygote is a putative first step for formation of the apical-basal axis of plants and is a fundamental feature of early embryogenesis and morphogenesis in angiosperms. Because fertilization and subsequent embryogenesis occur in embryo sacs, which are deeply embedded in ovular tissue, in vitro fertilization of isolated gametes is a powerful system to dissect mechanisms of fertilization and post-fertilization events. Rice is an emerging molecular and experimental model plant, however, profile of the first zygotic division within embryo sac and thus origin of apical-basal embryo polarity has not been closely investigated. Therefore, in the present study, the division pattern of rice zygote in planta was first determined accurately by observations employing serial sections of the egg apparatus, zygotes and two-celled embryos in the embryo sac. The rice zygote divides asymmetrically into a two-celled embryo consisting of a statistically significantly smaller apical cell with dense cytoplasm and a larger vacuolated basal cell. Moreover, detailed observations of division profiles of zygotes prepared by in vitro fertilization indicate that the zygote also divides into an asymmetric two-celled embryo as in planta. Such observations suggest that in vitro-produced rice zygotes and two-celled embryos may be useful as experimental models for further investigations into the mechanism and control of asymmetric division of plant zygotes.
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    • Publication Date:
      Date Created: 20100108 Date Completed: 20101214 Latest Revision: 20211020
    • Publication Date:
      20240829
    • Accession Number:
      10.1007/s00497-009-0129-9
    • Accession Number:
      20054584