Chain and conformation stability of solid-state DNA: implications for room temperature storage.

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  • Additional Information
    • Source:
      Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE
    • Publication Information:
      Publication: 1992- : Oxford : Oxford University Press
      Original Publication: London, Information Retrieval ltd.
    • Subject Terms:
      Preservation, Biological* ; Temperature*; DNA/*chemistry; 8-Hydroxy-2'-Deoxyguanosine ; Deoxyguanosine/analogs & derivatives ; Deoxyguanosine/analysis ; Hot Temperature ; Humidity ; Kinetics ; Nucleic Acid Conformation ; Water/chemistry
    • Abstract:
      There is currently wide interest in room temperature storage of dehydrated DNA. However, there is insufficient knowledge about its chemical and structural stability. Here, we show that solid-state DNA degradation is greatly affected by atmospheric water and oxygen at room temperature. In these conditions DNA can even be lost by aggregation. These are major concerns since laboratory plastic ware is not airtight. Chain-breaking rates measured between 70 degrees C and 140 degrees C seemed to follow Arrhenius' law. Extrapolation to 25 degrees C gave a degradation rate of about 1-40 cuts/10(5) nucleotides/century. However, these figures are to be taken as very tentative since they depend on the validity of the extrapolation and the positive or negative effect of contaminants, buffers or additives. Regarding the secondary structure, denaturation experiments showed that DNA secondary structure could be preserved or fully restored upon rehydration, except possibly for small fragments. Indeed, below about 500 bp, DNA fragments underwent a very slow evolution (almost suppressed in the presence of trehalose) which could end in an irreversible denaturation. Thus, this work validates using room temperature for storage of DNA if completely protected from water and oxygen.
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    • Accession Number:
      059QF0KO0R (Water)
      88847-89-6 (8-Hydroxy-2'-Deoxyguanosine)
      9007-49-2 (DNA)
      G9481N71RO (Deoxyguanosine)
    • Publication Date:
      Date Created: 20091209 Date Completed: 20100406 Latest Revision: 20211020
    • Publication Date:
      20240829
    • Accession Number:
      PMC2836546
    • Accession Number:
      10.1093/nar/gkp1060
    • Accession Number:
      19969539