Small RNAs and small proteins involved in resistance to cell envelope stress and acid shock in Escherichia coli: analysis of a bar-coded mutant collection.

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  • Author(s): Hobbs EC;Hobbs EC; Astarita JL; Storz G
  • Source:
    Journal of bacteriology [J Bacteriol] 2010 Jan; Vol. 192 (1), pp. 59-67.
  • Publication Type:
    Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 2985120R Publication Model: Print Cited Medium: Internet ISSN: 1098-5530 (Electronic) Linking ISSN: 00219193 NLM ISO Abbreviation: J Bacteriol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, DC : American Society for Microbiology
    • Subject Terms:
      Escherichia coli/*drug effects ; Escherichia coli/*metabolism ; Escherichia coli Proteins/*physiology ; Hydrochloric Acid/*pharmacology ; RNA, Bacterial/*physiology ; Sodium Dodecyl Sulfate/*pharmacology; Cell Wall/drug effects ; Cell Wall/metabolism ; Escherichia coli/genetics ; Escherichia coli Proteins/genetics ; Escherichia coli Proteins/metabolism ; Gene Expression Regulation, Bacterial/drug effects ; Gene Expression Regulation, Bacterial/genetics ; Genetic Complementation Test ; Kanamycin Resistance/physiology ; Mutation ; Oligonucleotide Array Sequence Analysis ; RNA, Bacterial/genetics ; Stress, Physiological
    • Abstract:
      More than 80 small regulatory RNAs (sRNAs) and 60 proteins of 16 to 50 amino acids (small proteins) are encoded in the Escherichia coli genome. The vast majority of the corresponding genes have no known function. We screened 125 DNA bar-coded mutants to identify novel cell envelope stress and acute acid shock phenotypes associated with deletions of genes coding for sRNAs and small proteins. Nine deletion mutants (ssrA, micA, ybaM, ryeF, yqcG, sroH, ybhT, yobF, and glmY) were sensitive to cell envelope stress and two were resistant (rybB and blr). Deletion mutants of genes coding for four small proteins (yqgB, mgrB, yobF, and yceO) were sensitive to acute acid stress. We confirmed each of these phenotypes in one-on-one competition assays against otherwise-wild-type lacZ mutant cells. A more detailed investigation of the SsrA phenotype suggests that ribosome release is critical for resistance to cell envelope stress. The bar-coded deletion collection we generated can be screened for sensitivity or resistance to virtually any stress condition.
    • Comments:
      Comment in: J Bacteriol. 2010 Jan;192(1):26-8. (PMID: 19854892)
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    • Grant Information:
      United States Intramural NIH HHS
    • Accession Number:
      0 (Escherichia coli Proteins)
      0 (RNA, Bacterial)
      368GB5141J (Sodium Dodecyl Sulfate)
      QTT17582CB (Hydrochloric Acid)
    • Publication Date:
      Date Created: 20090908 Date Completed: 20100105 Latest Revision: 20211020
    • Publication Date:
      20240829
    • Accession Number:
      PMC2798238
    • Accession Number:
      10.1128/JB.00873-09
    • Accession Number:
      19734312