Transient expression of a foreign gene by direct incorporation of DNA into intact plant tissue through vacuum infiltration.

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  • Author(s): Zhou B;Zhou B; Zhao X; Kawabata S; Li Y
  • Source:
    Biotechnology letters [Biotechnol Lett] 2009 Nov; Vol. 31 (11), pp. 1811-5. Date of Electronic Publication: 2009 Jul 18.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Kluwer Academic Publishers Country of Publication: Netherlands NLM ID: 8008051 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-6776 (Electronic) Linking ISSN: 01415492 NLM ISO Abbreviation: Biotechnol Lett Subsets: MEDLINE
    • Publication Information:
      Publication: 1999- : Dordrecht : Kluwer Academic Publishers
      Original Publication: [Kew, Eng., Science and Technology Letters]
    • Subject Terms:
      Gene Expression* ; Genes, Plant* ; Genetic Techniques*; DNA, Plant/*genetics ; Onions/*metabolism; Arabidopsis/enzymology ; Brassica rapa ; DNA, Recombinant/genetics ; Genetic Vectors/genetics ; Green Fluorescent Proteins/metabolism ; Plant Epidermis/cytology ; Subcellular Fractions/metabolism ; Transfection ; Vacuum
    • Abstract:
      We previously established a method to induce transient expression of foreign genes in intact plant tissue to detect the subcellular localization of proteins. Here, we have inserted a putative bZIP protein HY5 gene (SeqID: EU386772), isolated from the seedlings of turnips Brassica rapa L. subsp. rapa 'Tsuda,' and a receptor-like kinase gene AtRLK (SeqID: AY531551.1), isolated from Arabidopsis, into the plasmid pA7-GFP. We accomplished the direct incorporation of DNA into onion epidermal tissue by vacuum infiltration. By detecting GFP, which was fused with AtRLK or putative BrHY5, we determined that BrHY5 is located in the nucleus and AtRLK is located in the plasma membrane. This approach can be thus used to study the transient expression of foreign genes in intact tissue.
    • Accession Number:
      0 (DNA, Plant)
      0 (DNA, Recombinant)
      147336-22-9 (Green Fluorescent Proteins)
    • Publication Date:
      Date Created: 20090721 Date Completed: 20091204 Latest Revision: 20091008
    • Publication Date:
      20231215
    • Accession Number:
      10.1007/s10529-009-0080-8
    • Accession Number:
      19618274