Metabolic network analysis integrated with transcript verification for sequenced genomes.

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  • Additional Information
    • Source:
      Publisher: Nature Pub. Group Country of Publication: United States NLM ID: 101215604 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1548-7105 (Electronic) Linking ISSN: 15487091 NLM ISO Abbreviation: Nat Methods Subsets: MEDLINE
    • Publication Information:
      Original Publication: New York, NY : Nature Pub. Group, c2004-
    • Subject Terms:
      Genome, Protozoan* ; Models, Genetic* ; Transcription, Genetic*; Chlamydomonas reinhardtii/*metabolism ; Computational Biology/*methods ; Protozoan Proteins/*metabolism; Animals ; Chlamydomonas reinhardtii/enzymology ; Chlamydomonas reinhardtii/genetics ; Computer Simulation ; Enzymes/genetics ; Enzymes/metabolism ; Genetic Engineering ; Protozoan Proteins/genetics
    • Abstract:
      With sequencing of thousands of organisms completed or in progress, there is a growing need to integrate gene prediction with metabolic network analysis. Using Chlamydomonas reinhardtii as a model, we describe a systems-level methodology bridging metabolic network reconstruction with experimental verification of enzyme encoding open reading frames. Our quantitative and predictive metabolic model and its associated cloned open reading frames provide useful resources for metabolic engineering.
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    • Grant Information:
      P50 GM068763 United States GM NIGMS NIH HHS; P50 GM068763-06 United States GM NIGMS NIH HHS; P50 GM068763-08 United States GM NIGMS NIH HHS
    • Accession Number:
      0 (Enzymes)
      0 (Protozoan Proteins)
    • Publication Date:
      Date Created: 20090715 Date Completed: 20090813 Latest Revision: 20211020
    • Publication Date:
      20240829
    • Accession Number:
      PMC3139173
    • Accession Number:
      10.1038/nmeth.1348
    • Accession Number:
      19597503