Molecular cloning and characterization of the germline-restricted chromosome sequence in the zebra finch.

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  • Author(s): Itoh Y;Itoh Y; Kampf K; Pigozzi MI; Arnold AP
  • Source:
    Chromosoma [Chromosoma] 2009 Aug; Vol. 118 (4), pp. 527-36. Date of Electronic Publication: 2009 May 19.
  • Publication Type:
    Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Springer-Verlag Country of Publication: Austria NLM ID: 2985138R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0886 (Electronic) Linking ISSN: 00095915 NLM ISO Abbreviation: Chromosoma Subsets: MEDLINE
    • Publication Information:
      Original Publication: Wien : Springer-Verlag, 1947-
    • Subject Terms:
      Chromosomes/*genetics ; Cloning, Molecular/*methods ; Finches/*genetics; Animals ; Base Sequence ; Chromosome Mapping ; Female ; Genomic Library ; In Situ Hybridization, Fluorescence ; Male ; Molecular Sequence Data ; Ovary/metabolism ; Polymerase Chain Reaction ; Random Amplified Polymorphic DNA Technique ; Sequence Homology, Nucleic Acid ; Testis/metabolism
    • Abstract:
      The zebra finch (Taeniopygia guttata) germline-restricted chromosome (GRC) is the largest chromosome and has a unique system of transmission in germ cells. In the male, the GRC exists as a single heterochromatic chromosome in the germline and is eliminated from nuclei in late spermatogenesis. In the female, the GRC is bivalent and euchromatic and experiences recombination. These characteristics suggest a female-specific or female-beneficial function of the GRC. To shed light on the function of GRC, we cloned a portion of the GRC using random amplified polymorphic DNA-polymerase chain reaction and analyzed it using molecular genetic and cytogenetic methods. The GRC clone hybridized strongly to testis but not blood DNA in genomic Southern blots. In fluorescent in situ hybridization analysis on meiotic chromosomes from synaptonemal complex spreads, the probe showed hybridization across a large area of the GRC, suggesting that it contains repetitive sequences. We isolated a sequence homologous to the GRC from zebra finch chromosome 3 and a region of chicken chromosome 1 that is homologous to zebra finch chromosome 3; the phylogenetic analysis of these three sequences suggested that the GRC sequence and the zebra finch chromosome 3 sequence are most closely related. Thus, the GRC sequences likely originated from autosomal DNA and have evolved after the galliform-passeriform split. The present study provides a foundation for further study of the intriguing GRC.
    • References:
      Philos Trans R Soc Lond B Biol Sci. 1990 Aug 29;329(1253):109-14. (PMID: 1978357)
      Hum Reprod. 2006 Jun;21(6):1490-7. (PMID: 16484310)
      Brain Behav Evol. 1994;44(4-5):279-85. (PMID: 7842286)
      Genomics. 2006 Jan;87(1):181-90. (PMID: 16321505)
      Chromosome Res. 2002;10(2):101-8. (PMID: 11993930)
      Cytogenet Genome Res. 2007;119(1-2):105-12. (PMID: 18160789)
      Chromosoma. 1993 Feb;102(3):163-73. (PMID: 8458254)
      Genetica. 2001;111(1-3):319-28. (PMID: 11841177)
      Genome. 2004 Aug;47(4):732-41. (PMID: 15284878)
      Chromosome Res. 2008;16(6):839-49. (PMID: 18668333)
      Genome. 2006 Mar;49(3):269-74. (PMID: 16604110)
      Cytogenet Cell Genet. 1986;41(4):209-14. (PMID: 3709235)
      Annu Rev Genomics Hum Genet. 2008;9:109-27. (PMID: 18489256)
      J Mol Evol. 2000 Feb;50(2):154-69. (PMID: 10684349)
      J Biol. 2007;6(1):2. (PMID: 17352797)
      Brain Res Bull. 2002 Apr;57(6):737-49. (PMID: 12031270)
      Chromosome Res. 1998 Feb;6(2):105-13. (PMID: 9543013)
      Chromosoma. 1997 Oct;106(5):267-75. (PMID: 9297504)
      Am J Hum Genet. 2002 Dec;71(6):1353-68. (PMID: 12432495)
      Mol Gen Genet. 1997 Oct;256(3):252-6. (PMID: 9393449)
      Chromosome Res. 1999;7(4):289-95. (PMID: 10461874)
      Chromosoma. 2000;109(5):343-9. (PMID: 11007493)
      Chromosoma. 1998 Mar;107(1):17-32. (PMID: 9567198)
      BMC Genomics. 2008 Mar 18;9:131. (PMID: 18366674)
      Cytogenet Genome Res. 2002;98(2-3):210-5. (PMID: 12698006)
      Chromosome Res. 2001;9(4):283-99. (PMID: 11419793)
      Bioessays. 2001 Mar;23(3):242-50. (PMID: 11223881)
      BMC Biol. 2007 Sep 20;5:40. (PMID: 17883843)
      Chromosome Res. 2005;13(1):47-56. (PMID: 15791411)
      Chromosoma. 2005 Dec;114(6):403-9. (PMID: 16215738)
    • Grant Information:
      R01 DC000217 United States DC NIDCD NIH HHS; DC00217 United States DC NIDCD NIH HHS
    • Publication Date:
      Date Created: 20090520 Date Completed: 20091102 Latest Revision: 20211020
    • Publication Date:
      20250114
    • Accession Number:
      PMC2701497
    • Accession Number:
      10.1007/s00412-009-0216-6
    • Accession Number:
      19452161