[HBcAg-specific CTL response induced by PTD-HBcAg fusion protein to inhibit HBV replication in vitro].

Publisher: Xi bao yu fen zi mian yi xue za zhi bian ji bu Country of Publication: China NLM ID: 101139110 Publication Model: Print Cited Medium: Print ISSN: 1007-8738 (Print) Linking ISSN: 10078738 NLM ISO Abbreviation: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi Subsets: MEDLINE

Original Publication: Xiʹan : Xi bao yu fen zi mian yi xue za zhi bian ji bu

Hepatitis B Core Antigens/*immunology ; Hepatitis B virus/*immunology ; Recombinant Fusion Proteins/*immunology ; T-Lymphocytes/*immunology ; T-Lymphocytes, Cytotoxic/*immunology ; Virus Replication/*immunology; Animals ; Cell Line, Tumor ; Cell Proliferation ; Cells, Cultured ; Coculture Techniques ; Cytotoxicity Tests, Immunologic ; DNA, Viral/genetics ; DNA, Viral/metabolism ; Dendritic Cells/cytology ; Dendritic Cells/immunology ; Dendritic Cells/metabolism ; Enzyme-Linked Immunosorbent Assay ; Flow Cytometry ; Gene Products, tat/genetics ; Gene Products, tat/metabolism ; Hepatitis B Core Antigens/genetics ; Hepatitis B Core Antigens/metabolism ; Hepatitis B virus/genetics ; Hepatitis B virus/growth & development ; Humans ; Interferon-gamma/metabolism ; Interleukin-10/metabolism ; Interleukin-2/metabolism ; Interleukin-4/metabolism ; Mice ; Mice, Inbred BALB C ; Recombinant Fusion Proteins/metabolism ; T-Lymphocytes/cytology ; T-Lymphocytes/metabolism

Aim: To investigate the effects of specific cytotoxic T lymphocyte induced by sensitized dendritic cells(DCs)with PTD-HBcAg fusion protein on inhibiting HBV in vitro.
Methods: DCs were cultured and induced maturation by different fusion proteins, and cocultured with allogeneic T cells to detect the secretion level of IL-2, IL-4, IL-10 and INF-gamma in the supernatants of T cells by ELISA. Intracellular cytokine of proliferative T cells was analyzed by flow cytometry and the specific CTL activity was measured by a lactate dehydrogenase (LDH) release assay. The levels of HBsAg and HBV DNA in the supernatant of HepG2.2.15 cells were detected.
Results: Sensitized dendritic cells by different fusion proteins could promote cytokine secretion effectively, the levels of IL-2(552.7+/-117.5 ng/L) and INF-gamma(150.6+/-7.945 ng/L)induced by M-PTD-HBcAg were higher than that induced by M-HBcAg (420+/-12.47 ng/L and 107.5+/-12.19 ng/L, respectively).The amount of CTLs induced by PTD-HBcAg fusion protein was more than others by the analysis of intracellular cytokine of proliferative T cells. Specific CTL killing activity could be induced by PTD-HBcAg and HBcAg(P<0.05) and PTD-HBcAg significantly decreased the levels of HBsAg and HBV DNA.
Conclusion: Sensitised DCs by PTD-HBcAg fusion protein can stimulate cytokine secretion and increase cytotoxic T lymphocytes generation effectively, and also enhance the specific CTL activity to decrease the level of HBsAg and HBV DNA in supernatants of HepG2.2.15 cells.

0 (DNA, Viral)
0 (Gene Products, tat)
0 (Hepatitis B Core Antigens)
0 (Interleukin-2)
0 (Recombinant Fusion Proteins)
130068-27-8 (Interleukin-10)
207137-56-2 (Interleukin-4)
82115-62-6 (Interferon-gamma)

Date Created: 20090409 Date Completed: 20100308 Latest Revision: 20181201

20221213

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