Tumor morphology and immunohistochemical expression of estrogen receptor, progesterone receptor, p53, and Ki67 in urogenital carcinomas of California sea lions (Zalophus californianus).

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  • Author(s): Colegrove KM;Colegrove KM; Gulland FM; Naydan DK; Lowenstine LJ
  • Source:
    Veterinary pathology [Vet Pathol] 2009 Jul; Vol. 46 (4), pp. 642-55. Date of Electronic Publication: 2009 Mar 09.
  • Publication Type:
    Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Sage Country of Publication: United States NLM ID: 0312020 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1544-2217 (Electronic) Linking ISSN: 03009858 NLM ISO Abbreviation: Vet Pathol Subsets: MEDLINE
    • Publication Information:
      Publication: Jan. 2010- : Thousand Oaks, CA : Sage
      Original Publication: 1971- : Basel : New York : Karger.
    • Subject Terms:
      Sea Lions*; Carcinoma/*metabolism ; Carcinoma/*pathology ; Carcinoma/*veterinary ; Urogenital Neoplasms/*metabolism ; Urogenital Neoplasms/*pathology ; Urogenital Neoplasms/*veterinary; Animals ; California ; Estrogen Receptor alpha/metabolism ; Immunohistochemistry/veterinary ; Ki-67 Antigen/metabolism ; Receptors, Progesterone/metabolism ; Statistics, Nonparametric ; Tumor Suppressor Protein p53/metabolism
    • Abstract:
      Metastatic carcinoma of urogenital origin is a common cause of mortality in free-ranging California sea lions (Zalophus californianus). The etiology of this cancer is likely multifactorial, with viral infection, genetic factors, and exposure to environmental organochlorine contaminants possible contributing factors. In this study, expression of estrogen receptor alpha (ER alpha), progesterone receptor (PR), p53, and Ki67 were evaluated by immunohistochemistry in 12 sea lions with metastatic carcinoma, genital epithelial dysplasia, and intraepithelial neoplasia; 4 with genital epithelial dysplasia and intraepithelial neoplasia without metastases; and 6 control animals. Dysplastic and neoplastic lesions were identified in multiple areas of the cervix, vagina, penis, prepuce, and urethra in affected animals, suggesting multicentric development. Lesions were graded according to degree of epithelial dysplasia and infiltration and lesions of different grades were evaluated separately. Estrogen receptor expression was lower in intraepithelial lesions compared with normal genital epithelium, and expression in metastatic lesions was completely absent. There was progesterone receptor expression in neoplastic cells in intraepithelial lesions of all grades and in metastases, with no significant difference between lesion grades or between control and affected epithelium. Ki67 index and p53 expression increased with lesion grade and were higher in lesions than normal epithelium. Metastatic tumors exhibited highly variable morphology; however, proliferation index, ER alpha, PR, and p53 expression were similar in tumors with different patterns of growth. These results suggest that endogenous hormones, environmental contaminants that interact with steroid hormone receptors, and alterations in p53 may play a role in urogenital carcinogenesis in California sea lions.
    • Comments:
      Comment in: Vet Pathol. 2010 Jan;47(1):185; author reply 186. (PMID: 20080501)
    • Grant Information:
      ES007055 United States ES NIEHS NIH HHS
    • Accession Number:
      0 (Estrogen Receptor alpha)
      0 (Ki-67 Antigen)
      0 (Receptors, Progesterone)
      0 (Tumor Suppressor Protein p53)
    • Publication Date:
      Date Created: 20090312 Date Completed: 20091030 Latest Revision: 20100119
    • Publication Date:
      20240829
    • Accession Number:
      10.1354/vp.08-VP-0214-C-FL
    • Accession Number:
      19276047