Stimulatory and inhibitory effects of dimethyl sulfoxide and ethylene glycol on ATPase activity and calcium transport of sarcoplasmic membranes.

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  • Author(s): The R; Hasselbach W
  • Source:
    European journal of biochemistry [Eur J Biochem] 1977 Apr 15; Vol. 74 (3), pp. 611-21.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies Country of Publication: England NLM ID: 0107600 Publication Model: Print Cited Medium: Print ISSN: 0014-2956 (Print) Linking ISSN: 00142956 NLM ISO Abbreviation: Eur J Biochem Subsets: MEDLINE
    • Publication Information:
      Publication: -2004: Oxford, UK : Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
      Original Publication: Berlin, New York, Springer.
    • Subject Terms:
      Adenosine Triphosphatases/*metabolism ; Calcium/*metabolism ; Dimethyl Sulfoxide/*pharmacology ; Ethylene Glycols/*pharmacology ; Sarcoplasmic Reticulum/*metabolism; 4-Nitrophenylphosphatase/metabolism ; Animals ; Biological Transport ; Biological Transport, Active/drug effects ; Calcium/pharmacology ; Enzyme Activation/drug effects ; Kinetics ; Membrane Lipids/physiology ; Membranes/metabolism ; Membranes/ultrastructure ; Microscopy, Electron ; Muscles/metabolism ; Phospholipids/physiology ; Rabbits ; Sarcoplasmic Reticulum/ultrastructure
    • Abstract:
      1. The effect of dimethyl sulfoxide (Me2SO) and ethylene glycol on two different preparations of the sarcoplasmic reticulum, i.e. native membranes and membranes whose phospholipids were hydrolyzed by phospholipase A, were investigated using ATP and p-nitrophenylphosphate as substrates. 2. Me2SO and ethylene glycol inhibit both calcium-dependent ATP hydrolysis and ATP-supported calcium transport by native vesicles. 3. In contrast, calcium-dependent p-nitrophenylphosphatase activity as well as p-nitrophenyl-phosphate-supported calcium transport are activated by both agents at concentrations lower than 30% (v/v). 4. Me2SO strongly stimulates p-nitrophenylphosphate activity of vesicles treated with phospholipase A, but has relatively little effect on p-nitrophenylphosphatase activity of native vesicles. 5. Up to a concentration of approximately 40% Me2SO (v/v) the inhibiting effect on the calcium-dependent ATPase is fully reversible, but only partially reversible on calcium transport. 6. In the concentration range where Me2SO inhibits ATP hydrolysis and calcium transport, it does not affect ATP binding to the membranes nor calcium-dependent formation of phospho-protein. 7. The rate of dephosphorylation as well as the rate of Pi exchange between ATP and ADP are markedly reduced by the presence of 30% Me2SO (v/v). 8. While Me2SO inhibits passive calcium efflux, ethylene glycol produces a considerable activation. 9. ADP-dependent calcium efflux and ATP synthesis are activated by 15% Me2SO (v/v). Ethylene glycol reduces both activities. 10. The results suggest that the respective substrate-enzyme complexes are differently affected by the agents, resulting either in inhibition or stimulation
    • Accession Number:
      0 (Ethylene Glycols)
      0 (Membrane Lipids)
      0 (Phospholipids)
      EC 3.1.3.41 (4-Nitrophenylphosphatase)
      EC 3.6.1.- (Adenosine Triphosphatases)
      SY7Q814VUP (Calcium)
      YOW8V9698H (Dimethyl Sulfoxide)
    • Publication Date:
      Date Created: 19770415 Date Completed: 19770622 Latest Revision: 20190620
    • Publication Date:
      20240829
    • Accession Number:
      10.1111/j.1432-1033.1977.tb11430.x
    • Accession Number:
      192554