Sarcomere length dependence of power output is increased after PKA treatment in rat cardiac myocytes.

Publisher: American Physiological Society Country of Publication: United States NLM ID: 100901228 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0363-6135 (Print) Linking ISSN: 03636135 NLM ISO Abbreviation: Am J Physiol Heart Circ Physiol Subsets: MEDLINE

Original Publication: Bethesda, Md. : American Physiological Society,

Cell Shape* ; Muscle Strength*/drug effects ; Myocardial Contraction*/drug effects; Cyclic AMP-Dependent Protein Kinases/*metabolism ; Myocytes, Cardiac/*enzymology ; Myofibrils/*metabolism ; Sarcomeres/*enzymology; Adrenergic beta-Agonists/pharmacology ; Animals ; Calcium/metabolism ; Carrier Proteins/metabolism ; In Vitro Techniques ; Male ; Models, Cardiovascular ; Myocytes, Cardiac/drug effects ; Phosphorylation ; Rats ; Rats, Sprague-Dawley ; Sarcomeres/drug effects ; Troponin I/metabolism

The Frank-Starling relationship of the heart yields increased stroke volume with greater end-diastolic volume, and this relationship is steeper after beta-adrenergic stimulation. The underlying basis for the Frank-Starling mechanism involves length-dependent changes in both Ca(2+) sensitivity of myofibrillar force and power output. In this study, we tested the hypothesis that PKA-induced phosphorylation of myofibrillar proteins would increase the length dependence of myofibrillar power output, which would provide a myofibrillar basis to, in part, explain the steeper Frank-Starling relations after beta-adrenergic stimulation. For these experiments, adult rat left ventricles were mechanically disrupted, permeabilized cardiac myocyte preparations were attached between a force transducer and position motor, and the length dependence of loaded shortening and power output were measured before and after treatment with PKA. PKA increased the phosphorylation of myosin binding protein C and cardiac troponin I, as assessed by autoradiography. In terms of myocyte mechanics, PKA decreased the Ca(2+) sensitivity of force and increased loaded shortening and power output at all relative loads when the myocyte preparations were at long sarcomere length ( approximately 2.30 mum). PKA had less of an effect on loaded shortening and power output at short sarcomere length ( approximately 2.0 mum). These changes resulted in a greater length dependence of myocyte power output after PKA treatment; peak normalized power output increased approximately 20% with length before PKA and approximately 40% after PKA. These results suggest that PKA-induced phosphorylation of myofibrillar proteins explains, in part, the steeper ventricular function curves (i.e., Frank-Starling relationship) after beta-adrenergic stimulation of the left ventricle.

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K02-HL-71550 United States HL NHLBI NIH HHS; R01-HL-57852 United States HL NHLBI NIH HHS; T32-AR-048523 United States AR NIAMS NIH HHS

0 (Adrenergic beta-Agonists)
0 (Carrier Proteins)
0 (Troponin I)
0 (myosin-binding protein C)
EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
SY7Q814VUP (Calcium)

Date Created: 20090303 Date Completed: 20090619 Latest Revision: 20211020

20240829

PMC2685326

10.1152/ajpheart.00864.2008

19252095