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Chimeric enzyme composed of polyhydroxyalkanoate (PHA) synthases from Ralstonia eutropha and Aeromonas caviae enhances production of PHAs in recombinant Escherichia coli.
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- Additional Information
- Source:
Publisher: American Chemical Society Country of Publication: United States NLM ID: 100892849 Publication Model: Print Cited Medium: Internet ISSN: 1526-4602 (Electronic) Linking ISSN: 15257797 NLM ISO Abbreviation: Biomacromolecules Subsets: MEDLINE
- Publication Information:
Original Publication: Washington, DC : American Chemical Society, c2000-
- Subject Terms:
- Abstract:
Chimeric enzymes composed of polyhydroxyalkanoate (PHA) synthases from Ralstonia eutropha (Cupriavidus necator) (PhaC(Re)) and Aeromonas caviae (PhaC(Ac)) were constructed. PhaC(Re) is known for its potent enzymatic activity among the characterized PHA synthases. PhaCAc has broad substrate specificity and synthesizes short-chain-length (SCL)/medium-chain-length (MCL) PHA. We attempted to create chimeric enzymes inheriting both of the advantageous properties. Among eight chimeras, AcRe12, with 26% of the N-terminal of PhaC(Ac) and 74% of the C-terminal of PhaC(Re), exhibited comparable P(3-hydroxybutyrate) accumulation as parental enzymes in Escherichia coli JM109. Thus, AcRe12 was applied to SCL/MCL PHA production using E. coli LS5218 as the host. AcRe12 accumulated higher amount of PHA (50 wt %) than the parental enzymes. Furthermore, the PHA consisted of 2 mol % 3-hydroxyhexanoate as well as 3-hydroxybutyrate. Therefore, the chimeric PHA synthase, AcRe12, inherited the character of both of the parental enzymes and thus exhibits improved enzymatic properties.
- Accession Number:
0 (DNA Primers)
0 (DNA, Recombinant)
0 (Polyhydroxyalkanoates)
0 (Recombinant Fusion Proteins)
EC 2.3.- (Acyltransferases)
EC 2.3.1.- (poly(3-hydroxyalkanoic acid) synthase)
- Publication Date:
Date Created: 20090220 Date Completed: 20100201 Latest Revision: 20090416
- Publication Date:
20221213
- Accession Number:
10.1021/bm801386j
- Accession Number:
19226108
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