[Selection and identification of DNA aptamers against DC-SIGN].

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  • Author(s): Chen F;Chen F; Zeng J; Sun P; Pan Q; Zhang XL
  • Source:
    Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi] 2008 Dec; Vol. 24 (12), pp. 1133-6.
  • Publication Type:
    English Abstract; Journal Article
  • Language:
    Chinese
  • Additional Information
    • Source:
      Publisher: Xi bao yu fen zi mian yi xue za zhi bian ji bu Country of Publication: China NLM ID: 101139110 Publication Model: Print Cited Medium: Print ISSN: 1007-8738 (Print) Linking ISSN: 10078738 NLM ISO Abbreviation: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi Subsets: MEDLINE
    • Publication Information:
      Original Publication: Xiʹan : Xi bao yu fen zi mian yi xue za zhi bian ji bu
    • Subject Terms:
      Aptamers, Nucleotide/*metabolism ; Cell Adhesion Molecules/*metabolism ; Lectins, C-Type/*metabolism ; Receptors, Cell Surface/*metabolism ; SELEX Aptamer Technique/*methods; Animals ; Mice ; NIH 3T3 Cells ; Protein Binding
    • Abstract:
      Aim: To develop the high affinity DNA aptamers which can selectively recognize DC-SIGN protein as putative preventive reagents and drugs against infectious diseases.
      Methods: A technique of cell surface-SELEX (TECS-SELEX) was employed to screen the DNA aptamers recognizing DC-SIGN displayed on the surface of NIH3T3 cells. Some aptamers from the aptamer pools with the highest affinity were cloned. The binding affinities between the aptamers and target cells were measured by flow cytometry and ELISA. The values of IC(50); were determined to detect the toxicity of aptamers for cells by MTT method.
      Results: The 14th aptamer pool had the highest affinity and the binding rates between single aptamer (ZD8) and DC-SIGN protein were in a dose dependent manner. DC-SIGN antibody can competitively inhibit the binding reactions between the aptamers and their target cells. The selected aptamers have very low or no toxicity for hepatic cells.
      Conclusion: The selected DNA aptamers bound to DC-SIGN protein with high affinity and specificity have been successfully selected, and they can be used both as preventive reagents and drugs against infectious diseases, such as AIDS, hepatitis C and tuberculosis.
    • Accession Number:
      0 (Aptamers, Nucleotide)
      0 (Cell Adhesion Molecules)
      0 (DC-specific ICAM-3 grabbing nonintegrin)
      0 (Lectins, C-Type)
      0 (Receptors, Cell Surface)
    • Publication Date:
      Date Created: 20081211 Date Completed: 20100408 Latest Revision: 20081210
    • Publication Date:
      20221213
    • Accession Number:
      19068192