Lipopolysaccharide diversity evolving in Helicobacter pylori communities through genetic modifications in fucosyltransferases.

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  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science
    • Subject Terms:
      Evolution, Molecular* ; Genetic Variation*; Fucosyltransferases/*genetics ; Helicobacter pylori/*genetics ; Lipopolysaccharides/*genetics; Gastric Mucosa/microbiology ; Gastric Mucosa/pathology ; Helicobacter pylori/enzymology ; Humans ; O Antigens ; Selection, Genetic
    • Abstract:
      Helicobacter pylori persistently colonizes the gastric mucosa of half the human population. It is one of the most genetically diverse bacterial organisms and subvariants are continuously emerging within an H. pylori population. In this study we characterized a number of single-colony isolates from H. pylori communities in various environmental settings, namely persistent human gastric infection, in vitro bacterial subcultures on agar medium, and experimental in vivo infection in mice. The lipopolysaccharide (LPS) O-antigen chain revealed considerable phenotypic diversity between individual cells in the studied bacterial communities, as demonstrated by size variable O-antigen chains and different levels of Lewis glycosylation. Absence of high-molecular-weight O-antigen chains was notable in a number of experimentally passaged isolates in vitro and in vivo. This phenotype was not evident in bacteria obtained from a human gastric biopsy, where all cells expressed high-molecular-weight O-antigen chains, which thus may be the preferred phenotype for H. pylori colonizing human gastric mucosa. Genotypic variability was monitored in the two genes encoding alpha1,3-fucosyltransferases, futA and futB, that are involved in Lewis antigen expression. Genetic modifications that could be attributable to recombination events within and between the two genes were commonly detected and created a diversity, which together with phase variation, contributed to divergent LPS expression. Our data suggest that the surrounding environment imposes a selective pressure on H. pylori to express certain LPS phenotypes. Thus, the milieu in a host will select for bacterial variants with particular characteristics that facilitate adaptation and survival in the gastric mucosa of that individual, and will shape the bacterial community structure.
    • References:
      Mol Microbiol. 2000 Jun;36(6):1187-96. (PMID: 10931272)
      J Bacteriol. 2006 Jan;188(1):249-54. (PMID: 16352841)
      Infect Immun. 2000 Feb;68(2):937-41. (PMID: 10639467)
      Infect Immun. 2001 Dec;69(12):7832-8. (PMID: 11705965)
      Proc Natl Acad Sci U S A. 2001 Dec 4;98(25):14625-30. (PMID: 11724955)
      Biochemistry. 1996 Feb 20;35(7):2489-97. (PMID: 8652593)
      Adv Carbohydr Chem Biochem. 2001;57:99-158. (PMID: 11836945)
      DNA Repair (Amst). 2007 Sep 1;6(9):1285-96. (PMID: 17398168)
      Glycobiology. 2000 Jul;10(7):701-13. (PMID: 10910974)
      J Clin Microbiol. 1995 Apr;33(4):918-23. (PMID: 7790461)
      J Infect Dis. 2000 Mar;181(3):1089-95. (PMID: 10720535)
      Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2863-8. (PMID: 16477004)
      FEMS Immunol Med Microbiol. 2005 Jul 1;45(1):11-23. (PMID: 15949928)
      PLoS Genet. 2005 Oct;1(4):e43. (PMID: 16217547)
      Lett Appl Microbiol. 1998 Jun;26(6):399-403. (PMID: 9717308)
      J Biol Chem. 2003 Jun 13;278(24):21893-900. (PMID: 12676935)
      J Infect Dis. 2002 Feb 15;185(4):503-12. (PMID: 11865403)
      J Lab Clin Med. 1999 May;133(5):488-500. (PMID: 10235132)
      Gastroenterology. 1997 Oct;113(4):1091-8. (PMID: 9322503)
      J Biol Chem. 2007 Mar 30;282(13):9973-9982. (PMID: 17251184)
      J Clin Microbiol. 2005 Aug;43(8):4237-41. (PMID: 16081988)
      Nucleic Acids Res. 1992 Dec 11;20(23):6221-5. (PMID: 1361982)
      Infect Immun. 2005 Nov;73(11):7677-86. (PMID: 16239572)
      J Infect Dis. 1992 Jul;166(1):149-53. (PMID: 1607687)
      Microbiology (Reading). 2004 Apr;150(Pt 4):817-830. (PMID: 15073292)
      Int J Med Microbiol. 2007 Sep;297(5):307-19. (PMID: 17467335)
      Cancer Epidemiol Biomarkers Prev. 2000 Sep;9(9):981-5. (PMID: 11008919)
      Proc Natl Acad Sci U S A. 2001 Dec 4;98(25):14607-12. (PMID: 11717398)
      Mol Microbiol. 1999 Feb;31(4):1265-74. (PMID: 10096092)
      Annu Rev Microbiol. 2000;54:615-40. (PMID: 11018139)
      J Biol Chem. 2006 Mar 10;281(10):6385-94. (PMID: 16407247)
      Dig Dis Sci. 1999 Jan;44(1):102-7. (PMID: 9952230)
      Infect Immun. 1999 Oct;67(10):5361-6. (PMID: 10496917)
      J Clin Microbiol. 1995 Sep;33(9):2496-7. (PMID: 7494058)
      J Appl Microbiol. 1997 Jul;83(1):67-75. (PMID: 9246772)
      Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1515-9. (PMID: 7878011)
      J Clin Microbiol. 2007 Dec;45(12):4039-43. (PMID: 17942650)
      FEMS Immunol Med Microbiol. 1995 Feb;10(3-4):271-80. (PMID: 7773243)
      FASEB J. 2007 Nov;21(13):3458-67. (PMID: 17567566)
      Aliment Pharmacol Ther. 2001 Apr;15(4):493-503. (PMID: 11284778)
      Eur J Biochem. 2000 Jan;267(2):305-20. (PMID: 10632700)
      J Clin Microbiol. 1996 Sep;34(9):2196-200. (PMID: 8862584)
      Biochemistry. 2006 Jul 4;45(26):8108-16. (PMID: 16800635)
      J Infect Dis. 2001 Aug 1;184(3):315-21. (PMID: 11443557)
      FEMS Immunol Med Microbiol. 1999 May;24(1):79-90. (PMID: 10340716)
      Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15056-61. (PMID: 11742075)
      Infect Immun. 1998 Jan;66(1):70-6. (PMID: 9423841)
      J Biol Chem. 2000 Feb 18;275(7):4988-94. (PMID: 10671538)
      N Engl J Med. 2002 Oct 10;347(15):1175-86. (PMID: 12374879)
      Cell Microbiol. 2005 Jul;7(7):911-9. (PMID: 15953024)
      FASEB J. 2006 Jul;20(9):1534-6. (PMID: 16720729)
      J Infect Dis. 2000 Jan;181(1):273-82. (PMID: 10608776)
      J Exp Med. 2004 Oct 18;200(8):979-90. (PMID: 15492123)
      Infect Immun. 2005 Aug;73(8):4818-22. (PMID: 16040995)
      J Clin Microbiol. 1997 Feb;35(2):468-70. (PMID: 9003617)
      Infect Immun. 1996 Nov;64(11):4598-605. (PMID: 8890213)
      Proc Natl Acad Sci U S A. 2000 Dec 19;97(26):14668-73. (PMID: 11121067)
      Glycobiology. 2006 Dec;16(12):158R-184R. (PMID: 16973733)
      Gastroenterology. 1998 Nov;115(5):1113-22. (PMID: 9797366)
      Biochimie. 2001 Jul;83(7):565-73. (PMID: 11522384)
      Carbohydr Res. 2008 Aug 11;343(12):1952-65. (PMID: 18279843)
      J Biol Chem. 2005 Nov 4;280(44):36848-56. (PMID: 16150700)
      Nucleic Acids Res. 1992 Oct 11;20(19):5137-42. (PMID: 1408828)
      Nat Rev Microbiol. 2007 Jun;5(6):441-52. (PMID: 17505524)
      Nat Rev Microbiol. 2006 Nov;4(11):826-36. (PMID: 17041630)
      Infect Immun. 2003 Nov;71(11):6573-81. (PMID: 14573679)
      J Bacteriol. 1992 Feb;174(4):1370-7. (PMID: 1735724)
    • Accession Number:
      0 (Lipopolysaccharides)
      0 (O Antigens)
      EC 2.4.1.- (Fucosyltransferases)
    • Publication Date:
      Date Created: 20081202 Date Completed: 20090204 Latest Revision: 20211020
    • Publication Date:
      20221213
    • Accession Number:
      PMC2583950
    • Accession Number:
      10.1371/journal.pone.0003811
    • Accession Number:
      19043574