Reduced levels of recent thymic emigrants in acute myeloid leukemia patients.

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  • Author(s): Li Y;Li Y; Yin Q; Yang L; Chen S; Geng S; Wu X; Zhong L; Schmidt CA; Przybylski GK
  • Source:
    Cancer immunology, immunotherapy : CII [Cancer Immunol Immunother] 2009 Jul; Vol. 58 (7), pp. 1047-55. Date of Electronic Publication: 2008 Nov 19.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Springer Verlag Country of Publication: Germany NLM ID: 8605732 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0851 (Electronic) Linking ISSN: 03407004 NLM ISO Abbreviation: Cancer Immunol Immunother Subsets: MEDLINE
    • Publication Information:
      Publication: Berlin : Springer Verlag
      Original Publication: Berlin ; New York, NY : Springer International, c1982-
    • Subject Terms:
      Cell Movement*/genetics; Leukemia, Myeloid, Acute/*immunology ; Receptors, Antigen, T-Cell, alpha-beta/*immunology ; T-Lymphocytes/*immunology ; Thymus Gland/*immunology; Adolescent ; Adult ; Aged ; Child ; Child, Preschool ; Female ; Gene Frequency ; Humans ; Male ; Middle Aged ; Receptors, Antigen, T-Cell, alpha-beta/genetics ; Young Adult
    • Abstract:
      Background: T cell immunodeficiency is a common feature in cancer patients, which may relate to initiation and development of tumor. Our previous study showed skewed expression of T cell receptor beta variable region (TRBV) subfamilies and clonal expansion of T cells in leukemia patients. In the present study, in order to further characterize the T cell immunity in acute myeloid leukemia (AML) patients, the level of recent thymic emigrants (RTE) was analyzed.
      Materials and Methods: Quantitative analysis of signal joint T cell recombination excision circles (deltaRec-psiJalpha sjTRECs) was performed in peripheral blood mononuclear cells (PBMCs) by real-time PCR (TaqMan), and the analysis of 23 TRBV-BD1 sjTRECs was performed by semi-nested PCR. Eighty-eight cases with AML were selected for this study; ten AML cases in complete remission (AML-CR) and 38 healthy individuals served as controls.
      Results: The levels of deltaRec-psiJalpha sjTRECs in PBMCs and CD3+ T cells were significantly decreased in AML patients, compared with healthy individuals and in patients in completive remission. Also the frequency of 23 TRBV-BD1 sjTRECs, and the number of detectable TRBV subfamily sjTRECs were significantly lower in AML patients than in healthy individuals. Moreover, the sjTRECs numbers and the frequency of TRBV-BD1 sjTRECs showed a progressive linear decline with age in AML patients.
      Conclusions: The decreased numbers of universal (deltaRec-psiJalpha) and family-specific (TRBV-BD1) sjTRECs indicate that the severe T cell immunodeficiency in AML patients is associated with reduced levels of recent thymic emigrants. In patients achieving complete remission both sjTREC counts return to normal values indicating the recovery of thymic function. Better understanding of the mechanisms underlying persistent immunodeficiency in leukemia patients may lead to novel treatment strategies to enhance immune competence.
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    • Accession Number:
      0 (Receptors, Antigen, T-Cell, alpha-beta)
    • Publication Date:
      Date Created: 20081120 Date Completed: 20090515 Latest Revision: 20240426
    • Publication Date:
      20240426
    • Accession Number:
      PMC11031001
    • Accession Number:
      10.1007/s00262-008-0621-3
    • Accession Number:
      19018534