Glutamate transporter-mediated glutamate secretion in the mammalian pineal gland.

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  • Author(s): Kim MH;Kim MH; Uehara S; Muroyama A; Hille B; Moriyama Y; Koh DS
  • Source:
    The Journal of neuroscience : the official journal of the Society for Neuroscience [J Neurosci] 2008 Oct 22; Vol. 28 (43), pp. 10852-63.
  • Publication Type:
    Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Society for Neuroscience Country of Publication: United States NLM ID: 8102140 Publication Model: Print Cited Medium: Internet ISSN: 1529-2401 (Electronic) Linking ISSN: 02706474 NLM ISO Abbreviation: J Neurosci Subsets: MEDLINE
    • Publication Information:
      Publication: Washington, DC : Society for Neuroscience
      Original Publication: [Baltimore, Md.] : The Society, c1981-
    • Subject Terms:
      Amino Acid Transport System X-AG/*physiology ; Glutamic Acid/*metabolism ; Pineal Gland/*metabolism; Amino Acid Transport System X-AG/antagonists & inhibitors ; Animals ; Aspartic Acid/pharmacology ; Calcium/metabolism ; Cells, Cultured ; Cesium/pharmacology ; Chlorides/pharmacology ; Dose-Response Relationship, Drug ; Enzyme Inhibitors/pharmacology ; Excitatory Amino Acid Antagonists/pharmacology ; Exocytosis/drug effects ; Exocytosis/physiology ; Glutamic Acid/pharmacology ; In Vitro Techniques ; Kainic Acid/analogs & derivatives ; Kainic Acid/pharmacology ; Macrolides/pharmacology ; Magnesium/pharmacology ; Male ; Melatonin/metabolism ; Membrane Potentials/drug effects ; Membrane Potentials/physiology ; Pineal Gland/cytology ; Potassium Chloride/pharmacology ; Rats ; Rats, Sprague-Dawley
    • Abstract:
      Glutamate transporters are expressed throughout the CNS where their major role is to clear released glutamate from presynaptic terminals. Here, we report a novel function of the transporter in rat pinealocytes. This electrogenic transporter conducted inward current in response to L-glutamate and L- or D-aspartate and depolarized the membrane in patch-clamp experiments. Ca2+ imaging demonstrated that the transporter-mediated depolarization induced a significant Ca2+ influx through voltage-gated Ca2+ channels. The Ca2+ rise finally evoked glutamate exocytosis as detected by carbon-fiber amperometry and by HPLC. In pineal slices with densely packed pinealocytes, glutamate released from the cells effectively activated glutamate transporters in neighboring cells. The Ca2+ signal generated by KCl depolarization or acetylcholine propagated through several cell layers by virtue of the regenerative "glutamate-induced glutamate release." Therefore, we suggest that glutamate transporters mediate synchronized elevation of L-glutamate and thereby efficiently downregulate melatonin secretion via previously identified inhibitory metabotropic glutamate receptors in the pineal gland.
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    • Grant Information:
      R01 GM083913 United States GM NIGMS NIH HHS; R01 GM083913-33 United States GM NIGMS NIH HHS; 27307C0011 United States ES NIEHS NIH HHS; 27398C0011 United States ES NIEHS NIH HHS; 27305C0011 United States ES NIEHS NIH HHS; GM083913 United States GM NIGMS NIH HHS
    • Accession Number:
      0 (Amino Acid Transport System X-AG)
      0 (Chlorides)
      0 (Enzyme Inhibitors)
      0 (Excitatory Amino Acid Antagonists)
      0 (Macrolides)
      0 (benzyloxyaspartate)
      1KSV9V4Y4I (Cesium)
      30KYC7MIAI (Aspartic Acid)
      3KX376GY7L (Glutamic Acid)
      52497-36-6 (dihydrokainic acid)
      660YQ98I10 (Potassium Chloride)
      88899-55-2 (bafilomycin A1)
      GNR9HML8BA (cesium chloride)
      I38ZP9992A (Magnesium)
      JL5DK93RCL (Melatonin)
      SIV03811UC (Kainic Acid)
      SY7Q814VUP (Calcium)
    • Publication Date:
      Date Created: 20081024 Date Completed: 20081201 Latest Revision: 20240615
    • Publication Date:
      20240615
    • Accession Number:
      PMC2596195
    • Accession Number:
      10.1523/JNEUROSCI.0894-08.2008
    • Accession Number:
      18945893