Genetic and epigenetic stability of cryopreserved and cold-stored hops (Humulus lupulus L.).

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  • Author(s): Peredo EL;Peredo EL; Arroyo-García R; Reed BM; Revilla MA
  • Source:
    Cryobiology [Cryobiology] 2008 Dec; Vol. 57 (3), pp. 234-41. Date of Electronic Publication: 2008 Sep 11.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0006252 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2392 (Electronic) Linking ISSN: 00112240 NLM ISO Abbreviation: Cryobiology Subsets: MEDLINE
    • Publication Information:
      Publication: <2000- > : Amsterdam : Elsevier
      Original Publication: San Diego, CA : Academic Press
    • Subject Terms:
      Preservation, Biological*; Humulus/*genetics; Amplified Fragment Length Polymorphism Analysis ; Cold Temperature ; DNA Methylation ; DNA, Plant/genetics ; Epigenesis, Genetic ; Plant Shoots/genetics ; Polymorphism, Genetic ; Random Amplified Polymorphic DNA Technique
    • Abstract:
      Conventional cold storage and cryopreservation methods for hops (Humulus lupulus L.) are available but, to our knowledge, the genetic and epigenetic stability of the recovered plants have not been tested. This study analyzed 51 accessions of hop using the molecular techniques, Random Amplified DNA Polymorphism (RAPD) and Amplified Fragment Length Polymorphism (AFLP), revealing no genetic variation among greenhouse-grown controls and cold stored or cryopreserved plants. Epigenetic stability was evaluated using Methylation Sensitive Amplified Polymorphism (MSAP). Over 36% of the loci were polymorphic when the cold and cryo-treated plants were compared to greenhouse plants. The main changes were demethylation events and they were common to the cryopreserved and cold stored plants indicating the possible effect of the in vitro establishment process, an essential step in both protocols. Protocol-specific methylation patterns were also detected indicating that both methods produced epigenetic changes in plants following cold storage and cryopreservation.
    • Accession Number:
      0 (DNA, Plant)
    • Publication Date:
      Date Created: 20080930 Date Completed: 20090122 Latest Revision: 20081201
    • Publication Date:
      20221213
    • Accession Number:
      10.1016/j.cryobiol.2008.09.002
    • Accession Number:
      18822279