Three-dimensional structure of myosin subfragment-1 from electron microscopy of sectioned crystals.

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  • Author(s): Winkelmann DA;Winkelmann DA; Baker TS; Rayment I
  • Source:
    The Journal of cell biology [J Cell Biol] 1991 Aug; Vol. 114 (4), pp. 701-13.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Rockefeller University Press Country of Publication: United States NLM ID: 0375356 Publication Model: Print Cited Medium: Print ISSN: 0021-9525 (Print) Linking ISSN: 00219525 NLM ISO Abbreviation: J Cell Biol Subsets: MEDLINE
    • Publication Information:
      Original Publication: New York : Rockefeller University Press
    • Subject Terms:
      Myosin Subfragments/*ultrastructure; Actomyosin/ultrastructure ; Animals ; Chickens ; Microscopy, Electron ; Models, Molecular ; Muscles ; Myosin Subfragments/isolation & purification ; Myosins/ultrastructure ; Protein Conformation
    • Abstract:
      Image analysis of electron micrographs of thin-sectioned myosin subfragment-1 (S1) crystals has been used to determine the structure of the myosin head at approximately 25-A resolution. Previous work established that the unit cell of type I crystals of myosin S1 contains eight molecules arranged with orthorhombic space group symmetry P212121 and provided preliminary information on the size and shape of the myosin head (Winkelmann, D. A., H. Mekeel, and I. Rayment. 1985. J. Mol. Biol. 181:487-501). We have applied a systematic method of data collection by electron microscopy to reconstruct the three-dimensional (3D) structure of the S1 crystal lattice. Electron micrographs of thin sections were recorded at angles of up to 50 degrees by tilting the sections about the two orthogonal unit cell axes in sections cut perpendicular to the three major crystallographic axes. The data from six separate tilt series were merged to form a complete data set for 3D reconstruction. This approach has yielded an electron density map of the unit cell of the S1 crystals of sufficient detail. to delineate the molecular envelope of the myosin head. Myosin S1 has a tadpole-shaped molecular envelope that is very similar in appearance to the pear-shaped myosin heads observed by electron microscopy of rotary-shadowed and negatively stained myosin. The molecule is divided into essentially three morphological domains: a large domain on one end of the molecule corresponding to approximately 60% of the total molecular volume, a smaller central domain of approximately 30% of the volume that is separated from the larger domain by a cleft on one side of the molecule, and the smallest domain corresponding to a thin tail-like region containing approximately 10% of the volume. This molecular organization supports models of force generation by myosin which invoke conformational mobility at interdomain junctions within the head.
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    • Grant Information:
      R01 AR038454 United States AR NIAMS NIH HHS; AR35186 United States AR NIAMS NIH HHS; R01 AR035186 United States AR NIAMS NIH HHS; GM33050 United States GM NIGMS NIH HHS; AR38454 United States AR NIAMS NIH HHS
    • Accession Number:
      0 (Myosin Subfragments)
      9013-26-7 (Actomyosin)
      EC 3.6.4.1 (Myosins)
    • Publication Date:
      Date Created: 19910801 Date Completed: 19910917 Latest Revision: 20190508
    • Publication Date:
      20231215
    • Accession Number:
      PMC2289899
    • Accession Number:
      10.1083/jcb.114.4.701
    • Accession Number:
      1869586