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[Odontoblast-like cell phenotype differentiation of cranial neural crest stem cell in vivo].
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- Author(s): Jiang HB;Jiang HB; Tian WD; Liu L; Tang W; Zheng XH; Chen XZ
- Source:
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition [Sichuan Da Xue Xue Bao Yi Xue Ban] 2008 Mar; Vol. 39 (2), pp. 276-8, 282.
- Publication Type:
English Abstract; Journal Article; Research Support, Non-U.S. Gov't
- Language:
Chinese
- Additional Information
- Source:
Publisher: Sichuan da xue xue bao (yi xue ban) bian ji bu Country of Publication: China NLM ID: 101162609 Publication Model: Print Cited Medium: Print ISSN: 1672-173X (Print) Linking ISSN: 1672173X NLM ISO Abbreviation: Sichuan Da Xue Xue Bao Yi Xue Ban Subsets: MEDLINE
- Publication Information:
Original Publication: Chengdu Shi : Sichuan da xue xue bao (yi xue ban) bian ji bu, 2003-
- Subject Terms:
- Abstract:
Objective: To investigate the feasibility of tooth regeneration by seeding cranial neural crest stem cell (CNCSC) in vivo.
Methods: Cranial neural tubes, dissected from mouse E9 d, were explanted onto fibronectin-coated dishes. CNCSC emigrated from the explanted neural tubes, and were cultured in a free-serum medium containing modified DMEM/F12. CNCSC, induced by FGF8, BMP2, TGFbeta1 and dentin matrix non-collagen protein (DMNCP), were cultured with collagen/chitosan, and implanted into the subcutaneous part of immunodeficiency mouse. The expression of collagen I/dentin sialophosphoprotein (DSPP) was analyzed by immunocytochemistry.
Results: With the scaffolds destroying, columnar cells possessing polarized nuclei and matrix produced by cells were showed in some regions. Immunohistochemical staining demonstrated that collagen type I and DSPP were expressed throughout the cytoplasm and matrix produced by cells.
Conclusion: By tissue engineering approach, our experiments further verify the odontoblast-like cell phenotype differentiation of CNCSC in vivo.
- Accession Number:
0 (Collagen Type I)
0 (Extracellular Matrix Proteins)
0 (Phosphoproteins)
0 (Protein Precursors)
0 (Sialoglycoproteins)
0 (dentin sialophosphoprotein)
- Publication Date:
Date Created: 20080718 Date Completed: 20090227 Latest Revision: 20101118
- Publication Date:
20240829
- Accession Number:
18630702
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