Effect of hyperosmotic stimulation on aquaporins gene expression in chick kidney.

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  • Author(s): Sugiura K;Sugiura K; Aste N; Fujii M; Shimada K; Saito N
  • Source:
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology [Comp Biochem Physiol A Mol Integr Physiol] 2008 Oct; Vol. 151 (2), pp. 173-9. Date of Electronic Publication: 2008 Jun 22.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Elsevier Science Country of Publication: United States NLM ID: 9806096 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1531-4332 (Electronic) Linking ISSN: 10956433 NLM ISO Abbreviation: Comp Biochem Physiol A Mol Integr Physiol Subsets: MEDLINE
    • Publication Information:
      Original Publication: New York, : Elsevier Science, c1998-
    • Subject Terms:
      Chickens* ; Saline Solution, Hypertonic* ; Stimulation, Chemical*; Aquaporins/*metabolism ; Gene Expression Regulation/*physiology ; Kidney/*metabolism; Amino Acid Sequence ; Animals ; Aquaporins/genetics ; Base Sequence ; Body Weight ; Gene Expression Regulation/genetics ; Humans ; Male ; Molecular Sequence Data ; Osmotic Pressure ; Permeability ; RNA, Messenger/metabolism ; Rats ; Reverse Transcriptase Polymerase Chain Reaction/methods ; Water-Electrolyte Balance/physiology
    • Abstract:
      Birds can produce hyperosmotic urine, but their renal morphology differs from that of mammals. Recent studies in mammals, suggested that various aquaporins (AQPs) are present in the kidney and play crucial roles in urine production. To elucidate the role of AQPs in the avian kidney, we first examined for the presence of AQP1, 2, 3, 4, 7 and 9 mRNAs in the chick (Gallus gallus) kidney by RT-PCR analysis. Next, we quantified variations of AQPs mRNAs levels in chick kidney after hyperosmotic stimulation (water-deprivation or salt-loading) by real-time RT-PCR analysis. Our study showed that in addition to AQP1, 2, 3, 4 and 7, chick kidney also expressed AQP9 and that hyperosmotic stimulation induced changes in AQPs expression. In particular, water-deprivation increased AQP2 and AQP3 mRNAs levels, whereas salt-loading induced a significant increase in AQP1, AQP2 and AQP9 mRNAs levels. AQP4 and AQP7 mRNA levels were not affected by any hyperosmotic stimulation. Taken together, these results indicated that the presence of AQPs in chick kidney is similar to that in mammals, that the chick kidney has an additional AQP9 and that AQP1, 2, 3 and 9 may play a crucial but different role in water permeability in this organ.
    • Accession Number:
      0 (Aquaporins)
      0 (RNA, Messenger)
      0 (Saline Solution, Hypertonic)
    • Publication Date:
      Date Created: 20080716 Date Completed: 20081023 Latest Revision: 20080825
    • Publication Date:
      20221213
    • Accession Number:
      10.1016/j.cbpa.2008.06.014
    • Accession Number:
      18621138