Phase, amplitude, and polarization microscopy with a sampling field sensor.

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  • Author(s): Tumbar R;Tumbar R
  • Source:
    Applied optics [Appl Opt] 2008 Jul 01; Vol. 47 (19), pp. D96-109.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Optica Publishing Group Country of Publication: United States NLM ID: 0247660 Publication Model: Print Cited Medium: Print ISSN: 1559-128X (Print) Linking ISSN: 1559128X NLM ISO Abbreviation: Appl Opt Subsets: MEDLINE
    • Publication Information:
      Publication: Washington, DC : Optica Publishing Group
      Original Publication: Easton, Pa., Optical Society of America.
    • Subject Terms:
      Microscopy, Interference/*methods ; Microscopy, Polarization/*methods; Algorithms ; Animals ; Caenorhabditis elegans ; Cells, Cultured ; Cheek ; Equipment Design ; Humans ; Image Processing, Computer-Assisted ; Larva/metabolism ; Light ; Models, Statistical ; Models, Theoretical ; Reproducibility of Results
    • Abstract:
      I describe an improved implementation of a previously reported interferometric device, the sampling field sensor (SFS) [Appl. Opt.47, B32-B43 (2008)]. It provides X, Y, and XY shearing interferometric information simultaneously (space multiplexed) with amplitude and polarization information while using time-multiplexed phase shifting. Its simple common-path configuration makes it compact and vibration insensitive, as demonstrated by the ~lambda/125 phase estimation repeatability that was below the coherent noise floor (estimated at ~lambda/50). The SFS may be viewed as an efficient, robust and accurate full-field optical-digital interface, easy to integrate with traditional imaging systems. This is demonstrated by using the sensor as the focal plane array of a transmitted-light microscope in a straightforward setup using an illumination path polarization phase shifter. This work is focused on a qualitative demonstration and presents phase, amplitude, and polarization images of different types of human cheek cells and Caenorhabditiselegans larvae.
    • Publication Date:
      Date Created: 20080703 Date Completed: 20080825 Latest Revision: 20190703
    • Publication Date:
      20240829
    • Accession Number:
      10.1364/ao.47.000d96
    • Accession Number:
      18594585