Inhibition of NMDA-induced outward currents by interleukin-1beta in hippocampal neurons.

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  • Author(s): Zhang R;Zhang R; Yamada J; Hayashi Y; Wu Z; Koyama S; Nakanishi H
  • Source:
    Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2008 Aug 08; Vol. 372 (4), pp. 816-20. Date of Electronic Publication: 2008 Jun 02.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE
    • Publication Information:
      Publication: <2002- >: San Diego, CA : Elsevier
      Original Publication: New York, Academic Press.
    • Subject Terms:
      Membrane Potentials*; Hippocampus/*physiology ; Interleukin-1beta/*physiology ; Pyramidal Cells/*physiology ; Receptors, N-Methyl-D-Aspartate/*physiology; Animals ; Calcium/metabolism ; Calcium/pharmacology ; Cell Separation ; Hippocampus/cytology ; Hippocampus/drug effects ; Interleukin-1beta/pharmacology ; Mice ; Mice, Inbred C57BL ; N-Methylaspartate/pharmacology ; Potassium Channels/drug effects ; Potassium Channels/physiology ; Pyramidal Cells/drug effects ; Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors
    • Abstract:
      There is increasing evidence that a functional interaction exists between interleukin-1beta (IL-1beta) and N-methyl-D-aspartate (NMDA) receptors. The present study attempted to elucidate the effect of IL-1beta on the NMDA-induced outward currents in mechanically dissociated hippocampal neurons using a perforated patch recording technique. IL-1beta (30-100 ng/ml) inhibited the mean amplitude of the NMDA-induced outward currents that were mediated by charybdotoxin (ChTX)-sensitive Ca(2+)-activated K(+) (K(Ca)) channels. IL-1beta (100 ng/ml) also significantly increased the mean ratio of the NMDA-induced inward current amplitudes measured at the end to the beginning of a 20-s application of NMDA. In hippocampal neurons from acute slice preparations, IL-1beta significantly inhibited ChTX-sensitive K(Ca) currents induced by a depolarizing voltage-step. IL-1 receptor antagonist antagonized effects of IL-1beta. These results strongly suggest that IL-1beta increases the neuronal excitability by inhibition of ChTX-sensitive K(Ca) channels activated by Ca(2+) influx through both NMDA receptors and voltage-gated Ca(2+) channels.
    • Accession Number:
      0 (Interleukin-1beta)
      0 (Potassium Channels)
      0 (Receptors, N-Methyl-D-Aspartate)
      0 (charybdotoxin receptor)
      6384-92-5 (N-Methylaspartate)
      SY7Q814VUP (Calcium)
    • Publication Date:
      Date Created: 20080604 Date Completed: 20080724 Latest Revision: 20131121
    • Publication Date:
      20240829
    • Accession Number:
      10.1016/j.bbrc.2008.05.128
    • Accession Number:
      18519030