[CDR3 spectratyping and clonal expansion of peripheral blood T cells in a patient with beta-mediterranean anaemia following peripheral blood stem cell transplantation].

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  • Author(s): Yao XS;Yao XS; Zhou MQ; Ma L; Luo W; Feng XQ; Wen Q; Wang XN
  • Source:
    Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2008 Apr; Vol. 28 (4), pp. 529-32.
  • Publication Type:
    Case Reports; English Abstract; Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    Chinese
  • Additional Information
    • Source:
      Publisher: Nanfang yi ke da xue xue bao bian ji bu Country of Publication: China NLM ID: 101266132 Publication Model: Print Cited Medium: Print ISSN: 1673-4254 (Print) Linking ISSN: 16734254 NLM ISO Abbreviation: Nan Fang Yi Ke Da Xue Xue Bao Subsets: MEDLINE
    • Publication Information:
      Original Publication: Guangzhou : Nanfang yi ke da xue xue bao bian ji bu, 2005-
    • Subject Terms:
      Complementarity Determining Regions/*immunology ; Graft vs Host Disease/*immunology ; Peripheral Blood Stem Cell Transplantation/*methods ; Receptors, Antigen, T-Cell, alpha-beta/*immunology ; beta-Thalassemia/*immunology; Child ; Complementarity Determining Regions/genetics ; Graft vs Host Disease/etiology ; Humans ; Male ; Peripheral Blood Stem Cell Transplantation/adverse effects ; Receptors, Antigen, T-Cell, alpha-beta/genetics ; beta-Thalassemia/surgery
    • Abstract:
      Objective: To study the CDR3 spectratyping and clonal expansion of T cells in the peripheral blood mononuclear cells (PBMCs) of patients with beta-mediterranean anemia patient undergoing allogeneic peripheral blood stem cell (PBSC) transplantation.
      Methods: The total RNA was isolated from the PBMCs of a nine-year-old boy with beta-mediterranean anemia before and after PBSC transplantation, and at the time of occurrence of graft-versus-host disease (GVHD). The CDR3 length was analyzed using immunoscope technique, and the characteristics of the T cell receptor (TCR) on the T cells with clonal expansion were examined by gene sequencing.
      Results: The 24 TCR BV CDR3 repertoire showed Gaussian distribution in the PBMCs isolated before the transplantation, and some of the TCR BV family CDR3 showed skewing in the PBMCs isolated 23 days after transplantation and at the onset of GVHD (28 days after transplantation), suggesting the clonal expansion of the donor PBSCs.
      Conclusion: The host PBMCs show muti-clonal expansion 23 days after PBSC transplantation, and in the stage of GVHD, some of the TCR BV family T cells show significant monoclonal expansion. Analysis of TCR CDR3 spectratyping and the molecular characteristics of specific TCR may help evaluate the immune reconstitution following the transplantation and indicate specific treatment for potential GVHD.
    • Accession Number:
      0 (Complementarity Determining Regions)
      0 (Receptors, Antigen, T-Cell, alpha-beta)
    • Publication Date:
      Date Created: 20080523 Date Completed: 20090327 Latest Revision: 20080522
    • Publication Date:
      20231215
    • Accession Number:
      18495582