Comparison of four eluents in the recovery of indigenous viruses from raw sludge.

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  • Author(s): Sattar SA; Westwood JC
  • Source:
    Canadian journal of microbiology [Can J Microbiol] 1976 Oct; Vol. 22 (10), pp. 1586-9.
  • Publication Type:
    Comparative Study; Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Canadian Science Publishing Country of Publication: Canada NLM ID: 0372707 Publication Model: Print Cited Medium: Print ISSN: 0008-4166 (Print) Linking ISSN: 00084166 NLM ISO Abbreviation: Can J Microbiol Subsets: MEDLINE
    • Publication Information:
      Publication: 2011- : Ottawa, ON : Canadian Science Publishing
      Original Publication: Ottawa, Canada : National Research Council, [1954-
    • Subject Terms:
      Sewage* ; Water Microbiology*; Enterovirus/*isolation & purification ; Poliovirus/*isolation & purification ; Reoviridae/*isolation & purification; Animals ; Caseins ; Cattle ; Fetal Blood ; Lactalbumin ; Meat ; Methods ; Protein Hydrolysates ; Tissue Extracts
    • Abstract:
      The efficiency of 3% casein hydrolysate (CH), 3% lactalbumin hydrolysate (LH), 3% beef extract (BE), and 10% fetal calf serum (FCS) was compared for the recovery of viruses from raw sludge. CH and LH proved to be inefficient and were eliminated from the study after initial testing. In tests with 20 different samples of raw sludge, beef extract eluted virus in 15 (75%) and FCS revealed virus in 19 (95%) of the samples using BS-C-1 cells. That different eluents were not eluting different viruses from the same sample was shown by the serologic and electron-microscopic examination of 43% (18/42) of the isolates. The identified viruses included members of the entero- (coxsackie B, and polio) and reo-virus groups.
    • Accession Number:
      0 (Caseins)
      0 (Protein Hydrolysates)
      0 (Sewage)
      0 (Tissue Extracts)
      9013-90-5 (Lactalbumin)
    • Publication Date:
      Date Created: 19761001 Date Completed: 19770103 Latest Revision: 20190720
    • Publication Date:
      20221213
    • Accession Number:
      10.1139/m76-233
    • Accession Number:
      184901