Identification of DOCK4 and its splicing variant as PIP3 binding proteins.

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  • Additional Information
    • Source:
      Publisher: Published for the International Union of Biochemistry and Molecular Biology by Taylor & Francis Country of Publication: England NLM ID: 100888706 Publication Model: Print Cited Medium: Internet ISSN: 1521-6551 (Electronic) Linking ISSN: 15216543 NLM ISO Abbreviation: IUBMB Life Subsets: MEDLINE
    • Publication Information:
      Original Publication: London ; Philadelphia, PA : Published for the International Union of Biochemistry and Molecular Biology by Taylor & Francis, c1999-
    • Subject Terms:
      Alternative Splicing* ; Gene Expression Regulation* ; Mutation*; GTPase-Activating Proteins/*chemistry ; rac GTP-Binding Proteins/*chemistry; Carrier Proteins ; Cell Line ; Cloning, Molecular ; Exons ; Humans ; Microscopy, Confocal ; Models, Biological ; Protein Binding ; Protein Isoforms ; Transfection
    • Abstract:
      DOCK4, a member of DOCK180 family proteins, was originally identified as a product of a gene deleted during tumor progression. Although its tumor suppression properties have been reported, the regulation mechanism of this protein has not been fully elucidated. DOCK4 shares two conserved domains called as DHR-1 and DHR-2 domain as other members including DOCK180. Although DHR-1 in DOCK180 is reported to bind to PIP(3), whether that of DOCK4 exhibits similar function has yet not been examined. In a search for novel PIP(3) binding proteins by the PIP(3) analog beads binding assay, we found that DOCK4 and its novel splicing variant, whose exon1 and exon52 are different from the known one, bind to PIP(3). Binding assay using deletion mutants of DOCK4 revealed that the binding region falls into the DHR-1 domain. These results raise the possibility that DOCK4 may be regulated by PIP(3) to exert its function.
    • Accession Number:
      0 (Carrier Proteins)
      0 (DOCK1 protein, human)
      0 (DOCK4 protein, human)
      0 (GTPase-Activating Proteins)
      0 (Protein Isoforms)
      EC 3.6.5.2 (rac GTP-Binding Proteins)
    • Publication Date:
      Date Created: 20080507 Date Completed: 20080807 Latest Revision: 20080623
    • Publication Date:
      20221213
    • Accession Number:
      10.1002/iub.67
    • Accession Number:
      18459162