Monoglycerides and diglycerides synthesis in a solvent-free system by lipase-catalyzed glycerolysis.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Humana Press Country of Publication: United States NLM ID: 8208561 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-0291 (Electronic) Linking ISSN: 02732289 NLM ISO Abbreviation: Appl Biochem Biotechnol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Clifton, N.J. : Humana Press, c1981-
    • Subject Terms:
      Diglycerides/*chemistry ; Fatty Acids, Nonesterified/*chemistry ; Fungal Proteins/*chemistry ; Glycerol/*chemistry ; Lipase/*chemistry ; Monoglycerides/*chemistry ; Soybean Oil/*chemistry; Enzyme Activation ; Enzyme Stability ; Enzymes, Immobilized/chemistry ; Solvents/chemistry ; Substrate Specificity
    • Abstract:
      Five lipases were screened (Thermomyces lanuginosus free and immobilized forms, Candida antarctica B, Candida rugosa, Aspergillus niger, and Rhizomucor miehei) to study their ability to produce monoglycerides (MG) and diglycerides (DG) through enzymatic glycerolysis of soybean oil. Lipase from C. antarctica was further studied to verify the enzyme load (wt% of oil mass), the molar ratio glycerol/oil, and the water content (wt% of glycerol) on the glycerolysis reaction. The best DG and MG productions were in the range 45-48% and 28-30% (w/w, based on the total oil), respectively. Using immobilized lipases, the amount of free fatty acids (FFA) produced was about 5%. However, the amount of FFA produced when using free lipases, with 3.5% extra water in the system, is equivalent to the MG yield, about 23%. The extra water content provides a competition between hydrolysis and glycerolysis reactions, increasing the FFA production.
    • Accession Number:
      0 (Diglycerides)
      0 (Enzymes, Immobilized)
      0 (Fatty Acids, Nonesterified)
      0 (Fungal Proteins)
      0 (Monoglycerides)
      0 (Solvents)
      8001-22-7 (Soybean Oil)
      EC 3.1.1.3 (Lipase)
      PDC6A3C0OX (Glycerol)
    • Publication Date:
      Date Created: 20080419 Date Completed: 20081014 Latest Revision: 20131121
    • Publication Date:
      20240829
    • Accession Number:
      10.1007/s12010-008-8133-3
    • Accession Number:
      18421596