Enzymatic probe sonication as a tool for solid-liquid extraction for total selenium determination by electrothermal-atomic absorption spectrometry.

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  • Author(s): Vale G;Vale G; Pereira S; Mota A; Fonseca L; Capelo JL
  • Source:
    Talanta [Talanta] 2007 Nov 30; Vol. 74 (2), pp. 198-205. Date of Electronic Publication: 2007 Jun 07.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE
    • Publication Information:
      Publication: Amsterdam : Elsevier
      Original Publication: Oxford : Pergamon Press
    • Subject Terms:
      Sonication*; Peptide Hydrolases/*chemistry ; Selenium/*analysis ; Spectrophotometry, Atomic/*methods; Animals ; Microwaves ; Ostreidae/chemistry ; Picea/chemistry ; Plankton/chemistry ; Reference Standards ; Sensitivity and Specificity ; Solid Phase Extraction ; Subtilisins/chemistry ; Temperature ; Trypsin/chemistry
    • Abstract:
      A new fast and reproducible approach is described for the application of the enzymatic probe sonication (EPS) methodology [J.L. Capelo, P. Ximénez-Embún, Y. Madrid-Albarrán, C. Cámara, Anal. Chem. 76 (2004) 233-237] for total selenium determination by electrothermal atomic absorption spectrometry, ET-AAS. Ni(NO3)2 and Pd(NO3)2 were studied as matrix modifiers in conjunction with H2O2, being best results obtained with Pd(NO3)2 plus H2O2. The presence of H2O2 as matrix modifier increases up to 66% the time-life of the graphite tubes, by avoiding the building-up of carbonaceous residues. BCR-414 plankton and ERM-CE 278 mussel tissue reference materials were used for proof-of-the-methodology. Different enzymes, protease XIV, substilisin and trypsin were studied. The use of fresh enzyme was found critical. Good Se recoveries were obtained for oyster tissue, 111%; BCR-414 plankton, 106% and ERM-CE 278 mussel tissue, 93%, when protease XIV was used. Data regarding microwave digestion versus EPS methodology is also presented and discussed.
    • Accession Number:
      EC 3.4.- (Peptide Hydrolases)
      EC 3.4.21.- (Subtilisins)
      EC 3.4.21.4 (Trypsin)
      EC 3.4.99.- (protease IV)
      H6241UJ22B (Selenium)
    • Publication Date:
      Date Created: 20080329 Date Completed: 20080418 Latest Revision: 20131121
    • Publication Date:
      20240829
    • Accession Number:
      10.1016/j.talanta.2007.05.051
    • Accession Number:
      18371630